Li Yazhen, Wang Ruoliu, Lin Yugang, Han Bing, Wang Bo, Wang Shufang
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
Heilongjiang ZBD Pharmaceutical Co. Ltd, Hulin, China.
Biomed Chromatogr. 2020 Dec;34(12):e4964. doi: 10.1002/bmc.4964. Epub 2020 Sep 18.
Ginkgo biloba L. leaf (GBL) is one of the most commonly used medicinal plants in the world. Phenolic acids with biological activities have a relatively high content in G. biloba leaf extracts (GBE); therefore they are of great significance for the quality control of GBL, GBE and its preparations. However, there have been few studies focused on their analysis. In this work, 12 phenolic acids, including 11 phenolic acid glycosides, were identified by liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (LC-Q-TOF/MS). Then, a method combining enzymolysis with HPLC was established for quantification of phenolic acid glycosides. It was found that the aglycones of phenolic acid glycosides mainly comprised five phenolic acids: 2,4,6-trihydroxybenzoic acid, protocatechuic acid, p-hydroxybenzoic acid, vanillic acid and p-coumaric acid. The quantitative method was validated, and the correlation coefficient (0.9993-0.9999), recovery (≥88.4%), repeatability (≤0.8%), and inter-day precision (≤5.5%) were satisfactory. Finally, the contents of glycosides of five phenolic acids in GBL, GBE and GBE injection from different sources were determined by the developed method. The method was accurate, repeatable and practicable, which could be helpful for the quantification of phenolic acid glycosides in other products containing GBL or GBE.
银杏叶是世界上最常用的药用植物之一。具有生物活性的酚酸在银杏叶提取物（GBE）中含量相对较高；因此，它们对于银杏叶、GBE及其制剂的质量控制具有重要意义。然而，针对其分析的研究较少。在本研究中，通过液相色谱-四极杆飞行时间质谱联用仪（LC-Q-TOF/MS）鉴定出12种酚酸，其中包括11种酚酸糖苷。然后，建立了一种酶解与高效液相色谱相结合的方法用于定量酚酸糖苷。结果发现，酚酸糖苷的苷元主要包括5种酚酸：2,4,6-三羟基苯甲酸、原儿茶酸、对羟基苯甲酸、香草酸和对香豆酸。该定量方法经过验证，相关系数（0.9993 - 0.9999）、回收率（≥88.4%）、重复性（≤0.8%）和日间精密度（≤5.5%）均令人满意。最后，采用所建立的方法测定了不同来源的银杏叶、GBE及GBE注射液中5种酚酸糖苷的含量。该方法准确、可重复且实用，有助于对其他含有银杏叶或GBE的产品中的酚酸糖苷进行定量分析。
