School of Life Science and Technology, Harbin Normal University, Harbin, P.R. China.
Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore, Pakistan.
DNA Cell Biol. 2020 Oct;39(10):1850-1861. doi: 10.1089/dna.2020.5637. Epub 2020 Aug 13.
Strigolactones (SLs) are the major plant hormones that play important roles in regulating organ development and environmental stress tolerance in plants. Even though the SL-related genes have been identified and well characterized in some plants, the information of SL-related genes in soybean is not fully established yet, especially in response to salt and alkaline stresses. In this study, we identified nine SL biosynthetic genes that include two D27, two CCD7, two CCD8, and three MAX1, as well as seven SL signaling genes that comprised two D14, two MAX2, and three D53 in the soybean genome. We found that SL biosynthetic and signaling genes are evolutionary conserved among different species. Syntenic analysis of these genes revealed their location on nine chromosomes as well as the presence of 10 pairs of duplication genes. Moreover, plant hormone and stress-responsive elements were identified in the promoter regions of SL biosynthetic and signaling genes. By using reverse transcription quantitative real-time PCR, we confirmed that SL genes have different tissue expressions in roots, stems, and leaves. The expression profile of SL biosynthetic and signaling genes under salt and alkaline stresses further confirmed the regulatory roles of SL biosynthetic and signaling genes under stress. In conclusion, we identified and provided valuable information on the soybean SL biosynthetic and signaling genes, and established a foundation for further functional analysis of soybean SL-related genes in response to salt and alkaline stresses.
独脚金内酯（SLs）是一类重要的植物激素，在调控植物器官发育和环境胁迫耐受中发挥重要作用。尽管一些植物中的 SL 相关基因已被鉴定并得到很好的描述，但大豆中 SL 相关基因的信息尚未完全建立，特别是在响应盐和碱性胁迫方面。在本研究中，我们在大豆基因组中鉴定到了 9 个 SL 生物合成基因，包括 2 个 D27、2 个 CCD7、2 个 CCD8 和 3 个 MAX1，以及 7 个 SL 信号转导基因，包括 2 个 D14、2 个 MAX2 和 3 个 D53。我们发现 SL 生物合成和信号转导基因在不同物种中是保守的。这些基因的共线性分析揭示了它们在 9 条染色体上的位置以及 10 对重复基因的存在。此外，在 SL 生物合成和信号转导基因的启动子区还鉴定到了植物激素和应激响应元件。通过使用反转录定量实时 PCR，我们证实 SL 基因在根、茎和叶中有不同的组织表达。SL 生物合成和信号转导基因在盐和碱性胁迫下的表达谱进一步证实了 SL 生物合成和信号转导基因在胁迫下的调控作用。总之，我们鉴定并提供了大豆 SL 生物合成和信号转导基因的有价值信息，为进一步研究大豆 SL 相关基因在响应盐和碱性胁迫中的功能奠定了基础。
