MacGregor R R, Safford M, Shalit M
Infectious Diseases Section, University of Pennsylvania School of Medicine, Philadelphia.
J Infect Dis. 1988 Apr;157(4):682-9. doi: 10.1093/infdis/157.4.682.
Acute ethanol intoxication inhibits neutrophil delivery to sites of inflammation and, concomitantly, reduces the adhesion of neutrophils to surfaces. The effect of ethanol on several other neutrophil functions required for normal delivery are examined herein. Serum-free neutrophil suspensions showed normal resting adherence to endothelial monolayers in ethanol concentrations up to 1000 mg/dL, but when neutrophils were stimulated by 10(-6)M N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP) to induce hyperadherence, ethanol induced a dose-dependent inhibition that was significant at concentrations greater than or equal to 500 mg/dL. Pretreating the endothelium with ethanol had no effect. Similarly, resting surface expression of the adhesive glycoprotein Mac-1 was unaffected by ethanol, but its up-regulation induced by fMLP was inhibited by 25.5% at 250 mg of ethanol/dL and by 52.3% at 1000 mg/dL. Release of both primary and secondary granule contents after activation showed dose-dependent inhibition, whereas resting granule content and spontaneous release were unaffected. Passive neutrophil deformability was significantly enhanced in 500 mg of ethanol/dL. Thus, ethanol affects several neutrophil delivery functions normally activated by inflammatory stimuli.
急性乙醇中毒会抑制中性粒细胞向炎症部位的输送，同时降低中性粒细胞与表面的黏附。本文研究了乙醇对正常输送所需的其他几种中性粒细胞功能的影响。无血清中性粒细胞悬液在乙醇浓度高达1000mg/dL时对内皮细胞单层的静息黏附正常，但当中性粒细胞受到10(-6)M N-甲酰-L-蛋氨酰-L-亮氨酰-L-苯丙氨酸（fMLP）刺激以诱导高黏附时，乙醇会诱导剂量依赖性抑制，在浓度大于或等于500mg/dL时具有显著性。用乙醇预处理内皮细胞没有效果。同样，黏附糖蛋白Mac-1的静息表面表达不受乙醇影响，但其由fMLP诱导的上调在乙醇浓度为250mg/dL时被抑制25.5%，在1000mg/dL时被抑制52.3%。激活后初级和次级颗粒内容物的释放显示出剂量依赖性抑制，而静息颗粒内容物和自发释放不受影响。在乙醇浓度为500mg/dL时，中性粒细胞的被动变形能力显著增强。因此，乙醇会影响几种通常由炎症刺激激活的中性粒细胞输送功能。
