Semper A E, Fitzsimons R B, Shotton D M
Department of Zoology, University of Oxford, U.K.
J Neurol Sci. 1988 Jan;83(1):93-108. doi: 10.1016/0022-510x(88)90023-8.
Existing methods for the ultrastructural identification of fibre types in human skeletal muscle are fallible. This has prompted us to develop a reliable immunoelectron microscopic approach for the identification of human skeletal muscle fibre types. Here we report the unambiguous electron microscopic identification of human type 1 muscle fibres, achieved by combining cryoultramicrotomy with colloidal gold immunocytochemical labelling, using a monoclonal antibody (N0Q7.5.4D) which is specific for the heavy chain of the slow myosin isoform of human skeletal muscle. This method for the identification of muscle fibre types and determination of myosin isoform distributions may have important applications in the ultrastructural study of pathological muscle and in the analysis of myofibrillar assembly during myogenesis.
现有的用于超微结构鉴定人类骨骼肌纤维类型的方法存在误差。这促使我们开发一种可靠的免疫电子显微镜方法来鉴定人类骨骼肌纤维类型。在此，我们报告了通过将冷冻超薄切片术与胶体金免疫细胞化学标记相结合，利用一种对人类骨骼肌慢肌球蛋白异构体重链具有特异性的单克隆抗体（N0Q7.5.4D），实现了对人类1型肌纤维的明确电子显微镜鉴定。这种鉴定肌纤维类型和确定肌球蛋白异构体分布的方法可能在病理性肌肉的超微结构研究以及肌发生过程中肌原纤维组装的分析中具有重要应用。
