Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, Neyshabur, Iran.
Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Photodiagnosis Photodyn Ther. 2020 Dec;32:101929. doi: 10.1016/j.pdpdt.2020.101929. Epub 2020 Aug 11.
Near-infrared triggered photodynamic therapy (NIR-PDT) has been introduced as a relatively deep tumor treatment modality. The gold Nanoechinus (Au NE) is a rare type of nanostructures that act as a transducer to change NIR wavelength to ultraviolet (UV) and visible lights. During the photodynamic process, Au nanoechinus (Au NE) converts the irradiation of 980 nm to 674 nm which is absorbed by Zn(II) Phthalocyanine tetrasulfonic acid (ZnPcS). In this study the cooperation effect of Au NE and ZnPcS in PDT on MCF7 and Hela cells was investigated.
Cytotoxicity and phototoxicity of the composition having different concentrations of Au NE and ZnPcS upon irradiation of 980 nm NIR light were evaluated against MCF7 and Hela cells after two different incubation times and irradiating with two different power densities of laser.
Among different experimental groups, in MCF7 cells, which were incubated for 48 h with 50 μg/mL Au NE+2μM ZnPcS and were treated by 980 nm laser with a power density of 200 mW cm for 15 and 30 min, 48 and 38% cell viability were recorded. No appreciable result was observed due to PDT of Hela cells.
Comparing to other PDT modalities against MCF7 cells, NIR-PDT procedure suggested in this study with the synergistic effect of Au NE and ZnPcS could be a secure promising modality in the treatment of deep-seated tumors. Carefully increasing the power density and ambient temperature, to the extent of skin tolerance threshold value, seems to be efficient in the treatment of Hela cells.
近红外触发光动力疗法（NIR-PDT）已被引入作为一种相对较深的肿瘤治疗方式。金纳米海胆（Au NE）是一种特殊类型的纳米结构，可作为换能器将近红外波长转换为紫外线（UV）和可见光。在光动力过程中，金纳米海胆（Au NE）将 980nm 的辐射转换为 674nm，被锌（II）酞菁四磺酸（ZnPcS）吸收。在这项研究中，研究了 Au NE 和 ZnPcS 在 MCF7 和 Hela 细胞的 PDT 中的协同作用。
用不同浓度的 Au NE 和 ZnPcS 组成物，在两种不同的孵育时间和两种不同的激光功率密度下对 MCF7 和 Hela 细胞进行照射，评估其对细胞的细胞毒性和光毒性。
在不同的实验组中，在 MCF7 细胞中，孵育 48 小时，Au NE 浓度为 50μg/ml+ZnPcS 浓度为 2μM，用 980nm 激光照射，功率密度为 200mW/cm，照射时间为 15 和 30 分钟，记录到 48%和 38%的细胞活力。由于 Hela 细胞的 PDT 没有观察到明显的效果。
与针对 MCF7 细胞的其他 PDT 方式相比，本研究中提出的 NIR-PDT 方法与 Au NE 和 ZnPcS 的协同作用可能是一种安全有前途的深部肿瘤治疗方法。在不超过皮肤耐受阈值的情况下，小心地增加激光功率密度和环境温度，对 Hela 细胞的治疗似乎是有效的。
