Institute of Resource Biology and Biotechnology, Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
Food Funct. 2020 Sep 23;11(9):7762-7774. doi: 10.1039/d0fo00890g.
This study investigated the protective effects of maca ethanol extract (EEM) and N-(3-methozybenzyl)-(9Z,12Z,15Z)-octadecatrienamide (M 18:3) on corticosterone (CORT)-induced testicular toxicity. Male Wistar rats were divided into 5 groups. Except for the control group, CORT (40 mg per kg·bw) was injected subcutaneously for 21 consecutive days to induce testicular toxicity. 1 h before CORT injection, the rats were treated with EEM (400 mg per kg·bw) and M 18:3 (5 mg per kg·bw, 25 mg per kg·bw) by gavage, except for the control group and model group. Epididymal sperm and biochemical, and histological parameters were evaluated for the protective effects of the drugs. EEM (400 mg per kg·bw) and M 18:3 (5 mg per kg·bw, 25 mg per kg·bw) increased the sperm concentration and sperm motility, decreased the production of abnormal sperms, and increased the number of spermatogonia and primary spermatocytes in the seminiferous tubules of CORT-induced rats. Moreover, EEM and M 18:3 decreased the MDA levels and the positive expression rates of TUNEL, whereas they increased the activities of SOD, CAT, GSH-Px, and GST, and the contents of GSH in the testicles of CORT-induced rats. Furthermore, EEM and M 18:3 alleviated CORT-induced reduction in the positive expression rates of PCNA and Ki67 in the testicles of rats. Besides, EEM and M 18:3 reduced the expression levels of Keap-1 and increased the expression levels of Nrf2, HO-1, γ-GCS, and NQO1 in the testicles of CORT-induced rats. In summary, the protective effects of EEM and M 18:3 may be attributed to their anti-oxidative and anti-apoptotic properties.
本研究探讨了玛咖乙醇提取物（EEM）和 N-（3-甲氧基苄基）-（9Z,12Z,15Z）-十八碳三烯酰胺（M 18:3）对皮质酮（CORT）诱导的睾丸毒性的保护作用。雄性 Wistar 大鼠分为 5 组。除对照组外，连续 21 天每天皮下注射 CORT（40mg/kg·bw）诱导睾丸毒性。在 CORT 注射前 1h，除对照组和模型组外，其余各组大鼠分别灌胃给予 EEM（400mg/kg·bw）和 M 18:3（5mg/kg·bw、25mg/kg·bw）。评估药物的保护作用，包括附睾精子和生化、组织学参数。EEM（400mg/kg·bw）和 M 18:3（5mg/kg·bw、25mg/kg·bw）可提高精子浓度和精子活力，降低异常精子的产生，增加 CORT 诱导大鼠睾丸曲细精管中生精细胞和初级精母细胞的数量。此外，EEM 和 M 18:3 降低了 MDA 水平和 TUNEL 阳性表达率，增加了 SOD、CAT、GSH-Px 和 GST 的活性，以及 GSH 在 CORT 诱导大鼠睾丸中的含量。此外，EEM 和 M 18:3 缓解了 CORT 诱导的大鼠睾丸中 PCNA 和 Ki67 阳性表达率的降低。此外，EEM 和 M 18:3 降低了 Keap-1 的表达水平，增加了 Nrf2、HO-1、γ-GCS 和 NQO1 在 CORT 诱导大鼠睾丸中的表达水平。综上所述，EEM 和 M 18:3 的保护作用可能归因于其抗氧化和抗凋亡特性。
