Centre for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University in Prague and General University Hospital, Prague, Czech Republic.
J Clin Endocrinol Metab. 2020 Nov 1;105(11). doi: 10.1210/clinem/dgaa528.
Gestational diabetes mellitus (GDM) is accompanied by subclinical inflammation; however, little is known about local inflammation in adipose tissue and placenta.
To analyze systemic and local subclinical inflammation and adipose tissue lymphocyte content and phenotype in pregnant women with and without GDM.
Observational study.
Academic hospital.
Twenty-one pregnant women with GDM (GDM group), 16 pregnant women without GDM (non-GDM group) and 15 nonpregnant control women (N group).
Serum samples taken at 28 to 32 (visit 1 [V1]) and 36 to 38 (V2) gestational weeks and 6 to 12 months after delivery (V3) in the GDM and non-GDM group and before elective gynecological surgery in the N group. Subcutaneous (SAT) and visceral adipose tissue (VAT) obtained during cesarean delivery or surgery.
Serum levels and adipose tissue expression of proinflammatory cytokines, adipose tissue lymphocyte content and phenotype (for a subset of GDM and non-GDM subjects).
Accented proinflammatory state in GDM was documented by increased circulating tumor necrosis factor-α (TNF-α) levels. In both groups of pregnant females total lymphocytes were higher in VAT compared to SAT. In GDM subjects B cells and NKT cells were higher in SAT compared to VAT and T helper cells were increased relative to SAT of non-GDM group, while no intercompartmental adipose tissue differences were seen in non-GDM women.
Pregnant females had higher total lymphocyte count in VAT relative to SAT regardless of GDM. In addition to increased systemic subclinical inflammation, GDM was associated with significant differences in lymphocyte composition between subcutaneous and visceral adipose tissue depots.
妊娠糖尿病（GDM）伴有亚临床炎症；然而，人们对脂肪组织和胎盘的局部炎症知之甚少。
分析有和无 GDM 的孕妇的全身和局部亚临床炎症以及脂肪组织淋巴细胞含量和表型。
观察性研究。
学术医院。
21 名患有 GDM 的孕妇（GDM 组）、16 名无 GDM 的孕妇（非 GDM 组）和 15 名未怀孕的对照女性（N 组）。
在 GDM 和非 GDM 组的 28 至 32 周（访视 1 [V1]）和 36 至 38 周（V2）以及分娩后 6 至 12 个月（V3）时采集血清样本，在 N 组中在选择性妇科手术前采集。在剖宫产或手术期间获得皮下（SAT）和内脏脂肪组织（VAT）。
血清中促炎细胞因子的水平和脂肪组织的表达、脂肪组织中淋巴细胞的含量和表型（针对 GDM 和非 GDM 受试者的一部分）。
GDM 患者的循环肿瘤坏死因子-α（TNF-α）水平升高，证明存在明显的促炎状态。在两组孕妇中，VAT 中的总淋巴细胞数高于 SAT。在 GDM 受试者中，SAT 中的 B 细胞和 NKT 细胞高于 VAT，与非 GDM 组的 SAT 相比，T 辅助细胞增加，而非 GDM 女性的 SAT 之间则没有发现隔间脂肪组织差异。
无论是否患有 GDM，孕妇的 VAT 中的总淋巴细胞计数都高于 SAT。除了全身性亚临床炎症增加外，GDM 还与皮下和内脏脂肪组织库之间的淋巴细胞组成存在显著差异有关。
