蛋白质的冷冻干燥。

Freeze-Drying of Proteins.

机构信息

School of Medical Instrument and Food Engineering, Institute of Biothermal Science, Shanghai, China.

出版信息

Methods Mol Biol. 2021;2180:683-702. doi: 10.1007/978-1-0716-0783-1_37.

Abstract

Freeze-drying has become one of the most important processes for the preservation of biological products. This chapter provides protocols for freeze-drying of proteins and discusses the importance of formulation, cycle development, and validation. Specific formulations for stabilization of proteins are presented as well as advice on common problems with freeze-drying of proteins.

摘要

冷冻干燥已成为生物制品保存的最重要工艺之一。本章提供了蛋白质冷冻干燥的方案，并讨论了配方、工艺开发和验证的重要性。还介绍了用于稳定蛋白质的特定配方以及有关蛋白质冷冻干燥常见问题的建议。