Keskintepe Levent, Eroglu Ali
Sher Fertility Institute, Las Vegas, USA.
University of Nevada School of Medicine, Las Vegas, NV, USA.
Methods Mol Biol. 2021;2180:721-730. doi: 10.1007/978-1-0716-0783-1_39.
Long-term preservation of mammalian sperm at suprazero temperatures is desired to save storage and space costs, as well as to facilitate transport of preserved samples. This can be accomplished by the freeze-drying of sperm samples. Although freeze-drying results in immotile and membrane-compromised sperm, intracytoplasmic sperm injection (ICSI) can be used to introduce such an immotile sperm into an oocyte and thus start the fertilization process. So far, it has been shown that improved freeze-drying protocols preserve chromosomal integrity and oocyte-activating factor(s) in rodent and mammalian species at 4 °C for several years and at ambient temperature for up to 1 year depending on species, which permits shipping freeze-dried samples at ambient temperature. This chapter concisely reviews freeze-drying of mammalian sperm first and then presents a simple freeze-drying protocol.
为节省储存和空间成本，并便于运输保存的样本，人们期望在高于零摄氏度的温度下长期保存哺乳动物精子。这可以通过精子样本的冻干来实现。尽管冻干会导致精子失去活力且细胞膜受损，但卵胞浆内单精子注射（ICSI）可用于将这种失去活力的精子引入卵母细胞，从而启动受精过程。到目前为止，已经表明，改进的冻干方案可在4℃下保存啮齿动物和哺乳动物物种的染色体完整性和卵母细胞激活因子数年，在室温下根据物种不同可保存长达1年，这使得冻干样本能够在室温下运输。本章首先简要回顾哺乳动物精子的冻干，然后介绍一个简单的冻干方案。
