血清 A-激酶锚定蛋白 1 水平与胰岛素抵抗和体重指数呈负相关，在新诊断的 2 型糖尿病患者中略有下降。

Serum level of A-kinase anchoring protein 1, negatively correlated with insulin resistance and body mass index, decreases slightly in patients with newly diagnosed T2DM.

机构信息

Department of Endocrinology, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, Suzhou, China.

出版信息

Endokrynol Pol. 2020;71(5):411-417. doi: 10.5603/EP.a2020.0051. Epub 2020 Aug 14.

DOI:10.5603/EP.a2020.0051

PMID:32797476

Abstract

INTRODUCTION

At present, the number of people suffering from diabetes and obesity is increasing in China, and also all over the world. Researchers found that decreased expression of A-kinase anchoring protein 1 (AKAP1), which was thought to regulate the function and structure of mitochondria, might be related to these two diseases. However, as far as we know, there is no study about the changes of serum AKAP1 protein in these two diseases. Hence we conducted this experiment to study the relationship between serum levels of AKAP1 with T2DM and obesity.

MATERIAL AND METHODS

There were 261 subjects involved in the experiment, including 130 patients with newly diagnosed T2DM and 131 individuals with normal glucose tolerance (NGT). They were further divided into four groups as follows. Subjects with NGT and normal weight (NW) were assigned to the NGT+NW group, those with NGT but with overweight (OW) or obesity (OB) were assigned to the NGT+OW/OB group, and so on; the rest were divided into the T2DM+NW group and the T2DM+OW/OB group. Serum AKAP1 levels were tested by ELISA method and compared by T-test. Linear regression was applied to discuss independent factors of AKAP1. Multiple logistic regression was used to analyse the relationship between AKAP1 and the prevalence of T2DM.

RESULTS

Serum AKAP1 in the NGT+NW group was 1.74 ± 0.42 ng/mL, higher than that in the NGT+OW/OB group, at 1.59 ± 0.41 ng/mL (t = 2.114, p = 0.036), and the T2DM+OW/OB group, at 1.52 ± 0.36 ng/ml (t = 3.219, p = 0.002). A-kinase anchoring protein 1 in 130 subjects with T2DM was lower than that in subjects with NGT, 1.57 ± 0.35 ng/mL vs. 1.67 ± 0.42 ng/mL, t = 2.036, p = 0.043. Liner regression showed that insulin resistance (IR) and body mass index (BMI) were independent factors negatively related to AKAP1: b = -0.019 and -0.032, respectively. Compared to the highest tertile of AKAP1, the prevalence of T2DM was higher in the other two tertiles; OR was 2.207 (1.203, 4.050) and 2.051 (1.121, 3.753), respectively.

CONCLUSIONS

Serum AKAP1 level decreases slightly in patients with T2DM and obesity. Subjects with lower leve1s of serum AKAP1 are susceptible to T2DM.

摘要

简介

目前，中国乃至全世界的糖尿病和肥胖症患者人数都在增加。研究人员发现，一种被认为能调节线粒体功能和结构的蛋白激酶锚定蛋白 1（AKAP1）表达减少，可能与这两种疾病有关。然而，据我们所知，目前还没有关于这两种疾病患者血清 AKAP1 蛋白变化的研究。因此，我们进行了这项实验，以研究血清 AKAP1 水平与 T2DM 和肥胖之间的关系。

材料与方法

共有 261 名受试者参与了这项实验，其中包括 130 名新诊断为 T2DM 的患者和 131 名糖耐量正常（NGT）个体。他们进一步分为四组。NGT 且体重正常（NW）的受试者被分配到 NGT+NW 组，NGT 但超重（OW）或肥胖（OB）的受试者被分配到 NGT+OW/OB 组，以此类推；其余受试者被分为 T2DM+NW 组和 T2DM+OW/OB 组。采用 ELISA 法检测血清 AKAP1 水平，并采用 T 检验进行比较。采用线性回归探讨 AKAP1 的独立影响因素。采用多因素 logistic 回归分析 AKAP1 与 T2DM 患病率的关系。

结果

NGT+NW 组血清 AKAP1 为 1.74±0.42ng/ml，高于 NGT+OW/OB 组的 1.59±0.41ng/ml（t=2.114，p=0.036），也高于 T2DM+OW/OB 组的 1.52±0.36ng/ml（t=3.219，p=0.002）。130 例 T2DM 患者的 AKAP1 水平低于 NGT 患者，分别为 1.57±0.35ng/ml 和 1.67±0.42ng/ml，t=2.036，p=0.043。线性回归显示，胰岛素抵抗（IR）和体重指数（BMI）是与 AKAP1 呈负相关的独立因素：b=-0.019 和-0.032。与 AKAP1 最高三分位相比，其他两个三分位的 T2DM 患病率更高；OR 分别为 2.207（1.203，4.050）和 2.051（1.121，3.753）。