From the Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Exp Clin Transplant. 2020 Dec;18(7):757-762. doi: 10.6002/ect.2019.0242. Epub 2020 Aug 15.
Toll-like receptors are a crucial part of the innate immune system and have a pivotal role in the acquired immunity system. Studies have shown that Toll-like receptors 2 and 4 are important during the transplant process. Therefore, we analyzed the gene expression of Toll-like receptors 2 and 4 in cases of renal transplant rejection. We measured the messenger RNA expression levels of Toll-like receptors 2 and 4 in renal transplant rejection recipients compared with nonrejection recipients.
We enrolled 151 deceased-donor kidney transplant recipients, whom we divided into 2 groups: 101 nonrejection recipients and 50 recipients with acute allograft rejection. We collected 3 mL of blood (treated with ethylenediaminetetraacetic acid) from each patient. Ribonucleic acid extraction and complementary DNA synthesis were conducted for all samples, and the constructed complementary DNAs were used for real-time polymerase chain reaction analysis.
We measured gene expression levels of Toll-like receptors 2 and 4 in renal transplant recipients with acute allograft rejection and in recipients who did not experience acute renal allograft rejection, and the results showed that messenger RNA expression levels for both Toll-like receptors 2 and 4 were significantly increased in the acute rejection group compared with the nonrejection group.
Toll-like receptors 4 and 2 could increase the risk of acute rejection after renal transplant and could be defined as a risk factor for rejection. Further studies are recommended.
Toll 样受体是先天免疫系统的重要组成部分，在获得性免疫系统中具有关键作用。研究表明，Toll 样受体 2 和 4 在移植过程中很重要。因此，我们分析了肾移植排斥反应病例中 Toll 样受体 2 和 4 的基因表达。我们测量了肾移植排斥反应受者与非排斥受者的 Toll 样受体 2 和 4 的信使 RNA 表达水平。
我们纳入了 151 名已故供体肾移植受者，将其分为 2 组：101 名非排斥受者和 50 名急性同种异体移植排斥受者。我们从每位患者采集 3 毫升血液（用乙二胺四乙酸处理）。对所有样本进行核糖核酸提取和互补 DNA 合成，构建的互补 DNA 用于实时聚合酶链反应分析。
我们测量了急性同种异体移植排斥受者和未经历急性肾同种异体移植排斥受者的 Toll 样受体 2 和 4 的基因表达水平，结果显示，急性排斥组 Toll 样受体 2 和 4 的信使 RNA 表达水平均明显高于非排斥组。
Toll 样受体 4 和 2 可增加肾移植后急性排斥的风险，并可定义为排斥的危险因素。建议进一步研究。
