Thornton J, Howard S P, Buckley J T
Department of Biochemistry and Microbiology, University of Victoria, Canada.
Biochim Biophys Acta. 1988 Mar 25;959(2):153-9. doi: 10.1016/0005-2760(88)90026-4.
We have determined the nucleotide sequence of a gene encoding Aeromonas hydrophila phospholipid-cholesterol acyltransferase, an enzyme which shares many properties with mammalian lecithin:cholesterol acyltransferase. The derived amino acid sequence of the protein contains two regions which are homologous to the proposed active sites and binding sites of the plasma acyltransferase and to similar sequences in other interfacially acting lipolytic enzymes. The amino terminus is preceded by a typical 18 amino acid signal sequence. The protein, which is released into the culture supernatant by Aeromonas hydrophila, is confined to the periplasm of Escherichia coli.
我们已经确定了编码嗜水气单胞菌磷脂胆固醇酰基转移酶的基因的核苷酸序列，该酶与哺乳动物卵磷脂：胆固醇酰基转移酶具有许多共同特性。该蛋白质的推导氨基酸序列包含两个区域，它们与血浆酰基转移酶的假定活性位点和结合位点以及其他界面作用脂解酶中的类似序列同源。氨基末端之前有一个典型的18个氨基酸的信号序列。嗜水气单胞菌释放到培养上清液中的这种蛋白质局限于大肠杆菌的周质中。
