腺苷与大鼠离体胰岛胰岛素分泌的调节
Adenosine and the regulation of insulin secretion by isolated rat islets of Langerhans.
作者信息
Ismail N A, El Denshary E E, Montague W
出版信息
Biochem J. 1977 May 15;164(2):409-13. doi: 10.1042/bj1640409.
The effect of adenosine in insulin secretion and adenylate cyclase activity of rat islets of Langerhans was investigated. Adenosine inhibited insulin secretion stimulated by glucose, glucagon, prostaglandin E2, tolbutamine and theophylline. Adenosine decreased basal adenylate cyclase activity of the islets as well as that stimulated by glucagon prostaglandin E2 and GTP, although fluoride-stimulated activity was not affected. Neither insulin secretion nor adenylate cyclase activity of the islets was affected by adenine, AMP or ADP. The inhibitory effect of adenosine on adenylate cyclase activity was not altered by either phenoxybenzamine (alpha-adrenergic blocker) or propranolol (beta-adrenergic blocker), suggesting that the effect is not mediated through the adrenergic receptors of the islet cells. These results suggest that the intracellular concentration of adenosine in the beta-cell may play a role in regulating insulin secretion and that this effect may be mediated via alterations in the activity of adenylate cyclase in the beta-cell.
研究了腺苷对大鼠胰岛胰岛素分泌及腺苷酸环化酶活性的影响。腺苷抑制由葡萄糖、胰高血糖素、前列腺素E2、甲苯磺丁脲和茶碱刺激引起的胰岛素分泌。腺苷降低胰岛的基础腺苷酸环化酶活性以及由胰高血糖素、前列腺素E2和GTP刺激引起的活性，不过氟化物刺激的活性未受影响。腺嘌呤、AMP或ADP对胰岛的胰岛素分泌及腺苷酸环化酶活性均无影响。苯氧苄胺（α-肾上腺素能阻滞剂）或普萘洛尔（β-肾上腺素能阻滞剂）均未改变腺苷对腺苷酸环化酶活性的抑制作用，这表明该作用并非通过胰岛细胞的肾上腺素能受体介导。这些结果提示，β细胞内腺苷的浓度可能在调节胰岛素分泌中起作用，且这种作用可能通过β细胞中腺苷酸环化酶活性的改变来介导。
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