Miura M, Koyama I, Matsuzaki H, Sakagishi Y, Ikezawa H, Komoda T
Central Clinical Laboratories, Tokyo Metropolitan Police Hospital, Japan.
Clin Chim Acta. 1988 Jan 15;171(1):63-74. doi: 10.1016/0009-8981(88)90291-4.
We have re-evaluated the isolation and characteristics of human urinary alkaline phosphatases (ALPs). From the results of physicochemical properties and immunological identification, the urinary ALPs from healthy subjects and patients with hepatoma were found to be similar in nature to liver and/or bone-like ALP. In patients with chronic or acute nephritis, the ALPs contained a major band of kidney-like ALP with a minor band of bone and intestinal ALPs. However, the ALPs in pregnant women had not only liver and bone ALPs but also placental-like ALP. It is interesting that only bone-like ALP was detected in psychiatric patients administered chlorpromazine. In the conditions we investigated, the molecular sizes of the urinary ALPs were similar as those of original ALPs, except for the enzyme from renal failure. Moreover, the total activity of urinary ALP was closely related to the level of serum ALP, being in a ratio of 1/40. In general, urinary ALP may be derived from serum ALP by minor modification, suggesting that the identification of excreted ALP in urine is a good marker for disturbed organs in respective diseases.
我们重新评估了人尿碱性磷酸酶（ALP）的分离及特性。从物理化学性质和免疫学鉴定结果来看，健康受试者及肝癌患者尿液中的ALP在本质上与肝脏和/或骨样ALP相似。在慢性或急性肾炎患者中，其ALP包含一条主要的肾样ALP条带以及一条次要的骨和肠ALP条带。然而，孕妇尿液中的ALP不仅有肝脏和骨ALP，还有胎盘样ALP。有趣的是，在服用氯丙嗪的精神病患者尿液中仅检测到骨样ALP。在我们所研究的情况下，除了肾衰竭患者的酶之外，尿液中ALP的分子大小与原始ALP相似。此外，尿ALP的总活性与血清ALP水平密切相关，比例为1/40。一般来说，尿ALP可能是由血清ALP经过微小修饰而来，这表明尿液中排泄的ALP的鉴定是各疾病中器官功能紊乱的良好标志物。
