Liu Honggen, Yang Peiying, Li Xiaojiang, Jia Yingjie
Department of Oncology, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300000, P.R. China.
Oncol Lett. 2020 Oct;20(4):35. doi: 10.3892/ol.2020.11898. Epub 2020 Jul 22.
There are few studies on the role of ring finger protein (RNF)180 in non-small cell lung cancer (NSCLC). The present study investigated the expression of RNF180 in NSCLC and its associations with the clinical factors and prognosis of NSCLC. The mRNA and protein expression levels of RNF180 were detected via reverse transcription-quantitative PCR and western blotting. Methylation-specific PCR (MSP) analysis was utilized to detect the methylation of RNF180. RNF180 expression levels were analyzed via immunohistochemistry. The protein and mRNA expression levels of RNF180 were lower in NSCLC cell lines compared with in the non-tumor cell line. Immunohistochemistry revealed that 64 patients that were negative for RNF180, while MSP detection analysis demonstrated that 60 patients exhibited RNF180 promoter methylation. The methylation status of RNF180 was significantly associated with RNF180 expression level. Among all factors evaluated, logistic regression analysis indicated that only T stage was significantly associated with RNF180 expression. Cox multivariate analysis demonstrated that RNF180 expression was an independent predictor of overall survival in patients with NSCLC. Methylation in the promoter of RNF180 was shown to reduce its expression levels. In summary, low RNF180 expression levels were associated with poor biological behaviors, thus RNF180 expression level may be used as a clinical marker to predict the prognosis of patients with NSCLC.
关于无名指蛋白（RNF）180在非小细胞肺癌（NSCLC）中的作用的研究较少。本研究调查了RNF180在NSCLC中的表达及其与NSCLC临床因素和预后的关系。通过逆转录定量PCR和蛋白质印迹法检测RNF180的mRNA和蛋白质表达水平。利用甲基化特异性PCR（MSP）分析检测RNF180的甲基化情况。通过免疫组织化学分析RNF180的表达水平。与非肿瘤细胞系相比，NSCLC细胞系中RNF180的蛋白质和mRNA表达水平较低。免疫组织化学显示，64例患者RNF180呈阴性，而MSP检测分析表明，60例患者存在RNF180启动子甲基化。RNF180的甲基化状态与RNF180表达水平显著相关。在所有评估因素中，逻辑回归分析表明只有T分期与RNF180表达显著相关。Cox多因素分析表明，RNF180表达是NSCLC患者总生存的独立预测因素。RNF180启动子甲基化可降低其表达水平。总之，RNF180低表达水平与不良生物学行为相关，因此RNF180表达水平可作为预测NSCLC患者预后的临床标志物。
