Tabatabaei Mirakabad Fatemeh Sadat, Khoramgah Maryam Sadat, Tahmasebinia Foozhan, Darabi Shahram, Abdi Saeed, Abbaszadeh Hojjat Allah, Khoshsirat Shahrokh
Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Hearing Disorders Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Lasers Med Sci. 2020 Summer;11(3):299-304. doi: 10.34172/jlms.2020.50. Epub 2020 Jun 21.
Parkinson's disease (PD) is one of the most common neurodegenerative disorders. The neuroinflammation in the brain of PD patients is one of the critical processes in the immune pathogenesis of PD leading to the neural loss in the substantia nigra. Due to the anti-inflammatory effects of curcumin (CU) and low-level laser therapy (LLLT), we examined the protective effect of CU and LLLT on PC12 cells treated with 6-hydroxydopamine (6-OHDA) as a Parkinson model. PC12 cells were pretreated using various concentrations of 6-OHDA for 24 hours to induce oxidative and cellular damages. PC12-6-OHDA cells were co-treated with CU and LLLT. The effects of CU and LLLT on Bax/Bcl2 and LC3/ATG10 expression were analyzed by real-time PCR and cell viability was assessed by MTT assay. Cell A Software was used to calculate the length of the Neurite and cell body areas. The results of this study show that the combination of CU dose-dependently and LLLT has a significant neuroprotective effect on cells and cellular death significantly decreases by increasing CU concentration. CU+LLLT decreases Bax/Bcl2 ratio which is an indicator of apoptosis and it also rescued a decrease in LC3 and ATG10 expression in comparison with 6-OHDA group. This study shows that the combination of 5 μM CU and LLLT has the best neuroprotective effect on PC12 cells against 6-OHDA by decreasing the BAX/BCL2 ratio.
帕金森病（PD）是最常见的神经退行性疾病之一。PD患者大脑中的神经炎症是PD免疫发病机制中的关键过程之一，导致黑质神经细胞丢失。鉴于姜黄素（CU）和低强度激光疗法（LLLT）的抗炎作用，我们研究了CU和LLLT对用6-羟基多巴胺（6-OHDA）处理的PC12细胞的保护作用，以此作为帕金森模型。用不同浓度的6-OHDA预处理PC12细胞24小时，以诱导氧化和细胞损伤。对PC12-6-OHDA细胞进行CU和LLLT联合处理。通过实时PCR分析CU和LLLT对Bax/Bcl2和LC3/ATG10表达的影响，并通过MTT法评估细胞活力。使用Cell A软件计算神经突长度和细胞体面积。本研究结果表明，CU和LLLT联合用药对细胞具有显著的剂量依赖性神经保护作用，且随着CU浓度的增加，细胞死亡显著减少。CU+LLLT降低了作为细胞凋亡指标的Bax/Bcl2比值，与6-OHDA组相比，它还挽救了LC3和ATG10表达的下降。本研究表明，5μM CU与LLLT联合使用通过降低BAX/BCL2比值，对PC12细胞对抗6-OHDA具有最佳的神经保护作用。
