Mohsenifar Zhaleh, Fridoni Mohammadjavad, Ghatrehsamani Mahdi, Abdollahifar Mohammad-amin, Abbaszadeh Hojjatallah, Mostafavinia Atarodalsadat, Fallahnezhad Somaye, Asghari Mohammadali, Bayat Saba, Bayat Mohammad
Pathology Department, Ayatollah Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Anatomy, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Lasers Med Sci. 2016 May;31(4):721-32. doi: 10.1007/s10103-016-1916-9. Epub 2016 Mar 10.
Osteoporosis (OP) and osteoporotic fracture are major public health issues for society; the burden for the affected individual is also high. Previous studies have shown that pulsed wave low-level laser therapy (PW LLLT) has osteogenic effects. This study intended to evaluate the impacts of PW LLLT on the cortical bone of osteoporotic rats' tibias in two experimental models, ovariectomized and dexamethasone-treated. We divided the rats into four ovariectomized induced OP (OVX-d) and four dexamethasone-treated (glucocorticoid-induced OP, GIOP) groups. A healthy (H) group of rats was considered for baseline evaluations. At 14 weeks following ovariectomy, we subdivided the OVX-d rats into the following groups: (i) control which had OP, (ii) OVX-d rats treated with alendronate (1 mg/kg), (iii) OVX-d rats treated with LLLT, and (iv) OVX-d rats treated with alendronate and PW LLLT. The remaining rats received dexamethasone over a 5-week period and were also subdivided into four groups: (i) control rats treated with intramuscular (i.m.) injections of distilled water (vehicle), (ii) rats treated with subcutaneous alendronate injections (1 mg/kg), (iii) laser-treated rats, and (iv) rats simultaneously treated with laser and alendronate. The rats received alendronate for 30 days and underwent PW LLLT (890 nm, 80 Hz, 0.972 J/cm(2)) three times per week during 8 weeks. Then, the right tibias were extracted and underwent a stereological analysis of histological parameters and real-time polymerase chain reaction (RT-PCR). A significant increase in cortical bone volume (mm(3)) existed in all study groups compared to the healthy rats. There were significant decreases in trabecular bone volume (mm(3)) in all study groups compared to the group of healthy rats. The control rats with OP and rats from the vehicle group showed significantly increased osteoclast numbers compared to most other groups. Alendronate significantly decreased osteoclast numbers in osteoporotic rats. Concurrent treatments (compounded by PW LLLT and alendronate) produce the same effect on osteoporotic bone.
骨质疏松症（OP）和骨质疏松性骨折是社会面临的重大公共卫生问题；对受影响个体而言负担也很重。先前的研究表明，脉冲波低强度激光疗法（PW LLLT）具有成骨作用。本研究旨在评估PW LLLT在去卵巢和地塞米松处理这两种实验模型中对骨质疏松大鼠胫骨皮质骨的影响。我们将大鼠分为四个去卵巢诱导骨质疏松（OVX - d）组和四个地塞米松处理组（糖皮质激素诱导的骨质疏松症，GIOP）。设立一个健康（H）组大鼠作为基线评估对象。去卵巢14周后，我们将OVX - d大鼠再细分为以下几组：（i）患有OP的对照组，（ii）用阿仑膦酸钠（1毫克/千克）处理的OVX - d大鼠，（iii）用LLLT处理的OVX - d大鼠，以及（iv）用阿仑膦酸钠和PW LLLT处理的OVX - d大鼠。其余大鼠在5周内接受地塞米松处理，也被细分为四组：（i）肌肉注射蒸馏水（赋形剂）的对照大鼠，（ii）皮下注射阿仑膦酸钠（1毫克/千克）的大鼠，（iii）激光处理的大鼠，以及（iv）同时接受激光和阿仑膦酸钠处理的大鼠。大鼠接受阿仑膦酸钠治疗30天，并在8周内每周接受三次PW LLLT（890纳米，80赫兹，0.972焦耳/平方厘米）。然后，取出右侧胫骨并对组织学参数进行体视学分析以及实时聚合酶链反应（RT - PCR）。与健康大鼠相比，所有研究组的皮质骨体积（立方毫米）均显著增加。与健康大鼠组相比，所有研究组的小梁骨体积（立方毫米）均显著减少。与大多数其他组相比，患有OP的对照大鼠和赋形剂组大鼠的破骨细胞数量显著增加。阿仑膦酸钠显著降低了骨质疏松大鼠的破骨细胞数量。联合治疗（PW LLLT和阿仑膦酸钠联合使用）对骨质疏松骨产生相同的效果。
