Department of Pathology, West China Hospital, Sichuan University, No. 37, Guo Xue Xiang, Chengdu, 610041, Sichuan, China.
Laboratory of Pathology, Clinical Research Centre for Breast, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.
Int J Clin Oncol. 2020 Dec;25(12):2025-2034. doi: 10.1007/s10147-020-01769-9. Epub 2020 Aug 16.
microRNAs, which expound the transcriptional regulation of gene expression, have been validated as prognostic markers in many tumors. The deregulated expression of microRNAs has been shown to aid classification of tumors and predict outcome in many tumors including breast PTs. The aim of our study is to investigate the clinical significance and prognostic value of microRNAs in PTs to identify a biomarker which has the potential for predicting prognosis and target therapy.
Quantitative real-time PCR (qRT-PCR) was used to detect the expression level of microRNA20b in 123 breast PTs patients. The correlations between the expression of microRNA20b and clinicopathological parameters were investigated. The prognostic significance of microRNA20b was investigated by the Kaplan-Meier survival and Cox proportional hazards regression model.
The expression level of microRNA20b increased with the increase in the tumor grade (p < 0.05). High expression of microRNA20b correlated with stromal overgrowth, marked stromal cellularity, high atypia of stromal cells, infiltrative tumor margin, high mitotic activity, tumor grade, local recurrence and metastasis (p < 0.05). High expression of microRNA20b correlated with the shorter disease-free survival (DFS) (log-rank test, p < 0.001). Multivariate Cox regression analysis showed that microRNA20b was an independent prognostic indicator for breast PTs patients.
The study demonstrated the promising potential of applying microRNA20b as a prognostic biomarker in PT patients.
microRNAs 阐述了基因表达的转录调控，已在许多肿瘤中被验证为预后标志物。microRNAs 的失调表达已被证明有助于许多肿瘤（包括乳腺 PTs）的分类，并预测结局。我们的研究旨在探讨 microRNAs 在 PTs 中的临床意义和预后价值，以确定一种具有预测预后和靶向治疗潜力的生物标志物。
采用定量实时 PCR（qRT-PCR）检测 123 例乳腺 PTs 患者 microRNA20b 的表达水平。研究 microRNA20b 的表达与临床病理参数之间的相关性。通过 Kaplan-Meier 生存和 Cox 比例风险回归模型探讨 microRNA20b 的预后意义。
microRNA20b 的表达水平随肿瘤分级的增加而升高（p<0.05）。microRNA20b 的高表达与间质过度生长、间质细胞丰富度高、间质细胞异型性高、浸润性肿瘤边缘、高有丝分裂活性、肿瘤分级、局部复发和转移相关（p<0.05）。microRNA20b 的高表达与无病生存（DFS）较短相关（log-rank 检验，p<0.001）。多因素 Cox 回归分析显示，microRNA20b 是乳腺 PTs 患者的独立预后指标。
该研究表明 microRNA20b 作为 PT 患者预后生物标志物具有很大的应用潜力。
