Warren Alpert Medical School at Brown University, Providence, RI, USA.
Department of Pediatrics, Women and Infants Hospital, Providence, RI, USA.
Reprod Sci. 2021 Jan;28(1):228-236. doi: 10.1007/s43032-020-00251-1. Epub 2020 Aug 17.
Preterm birth is a leading cause of infant morbidity and mortality. Decorin and biglycan are proteoglycans that play key roles in maintaining the connective tissue matrix and tensile strength of human fetal membranes and have been previously linked to PPROM. Extracellular matrix proteins, such as matrix metalloproteinase 2 (MMP-2), matrix metalloproteinase 9 (MMP-9), TIMP metallopeptidase inhibitor 1 (TIMP-1), TIMP metallopeptidase inhibitor 2 (TIMP-2), and collagen VI (COL-6), have also been linked to PPROM and may have utility in a serum-based screening model for this condition. To define the natural course of serum decorin and biglycan expression throughout the duration of healthy pregnancy, to explore patterns of serum decorin and biglycan expression in serum of asymptomatic women who go on to develop spontaneous preterm labor, and to investigate the potential role for matrix metalloproteinases, their inhibitors, and collagen VI in a serum-based screening model to predict PPROM. Serum decorin level decreases less than 1% per week, and serum biglycan decreases by 2.9% per week over the duration of healthy pregnancy. Serum decorin and biglycan concentrations do not differ in spontaneous preterm labor cases compared with those in controls. Mean concentrations of MMP-2, MMP-9, TIMP-1, TIMP-2, and COL-6 do not differ in PPROM cases compared with those in controls. We have demonstrated that serum decorin and biglycan concentrations remain stable throughout the duration of normal pregnancy and are not early indicators of preterm labor, while common MMPs, TIMPs, and collagen VI are not early indicators of PPROM.
早产是婴儿发病率和死亡率的主要原因。核心蛋白聚糖和 biglycan 是蛋白聚糖，在维持人类胎膜的结缔组织基质和拉伸强度方面发挥着关键作用，并且先前与 PPROM 有关。细胞外基质蛋白，如基质金属蛋白酶 2 (MMP-2)、基质金属蛋白酶 9 (MMP-9)、金属蛋白酶抑制剂 1 (TIMP-1)、金属蛋白酶抑制剂 2 (TIMP-2)和胶原 VI (COL-6)，也与 PPROM 有关，并且在基于血清的筛查模型中可能具有实用价值。为了定义健康妊娠期间血清核心蛋白聚糖和 biglycan 表达的自然过程，探讨无症状妇女发生自发性早产时血清核心蛋白聚糖和 biglycan 表达模式，并研究基质金属蛋白酶、其抑制剂和胶原 VI 在基于血清的筛查模型中预测 PPROM 的潜在作用。血清核心蛋白聚糖水平每周下降不到 1%，而血清 biglycan 每周下降 2.9%，持续健康妊娠期间。自发性早产病例与对照组相比，血清核心蛋白聚糖和 biglycan 浓度没有差异。PPROM 病例与对照组相比，MMP-2、MMP-9、TIMP-1、TIMP-2 和 COL-6 的平均浓度没有差异。我们已经证明，血清核心蛋白聚糖和 biglycan 浓度在正常妊娠期间保持稳定，不是早产的早期指标，而常见的 MMPs、TIMPs 和胶原 VI 不是 PPROM 的早期指标。
