Department of Molecular Biology and Biochemistry, Rutgers the State University of New Jersey, Piscataway, United States.
Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, United States.
Elife. 2020 Aug 17;9:e54309. doi: 10.7554/eLife.54309.
Nuclear RNAi provides a highly tractable system to study RNA-mediated chromatin changes and epigenetic inheritance. Recent studies have indicated that the regulation and function of nuclear RNAi-mediated heterochromatin are highly complex. Our knowledge of histone modifications and the corresponding histonemodifying enzymes involved in the system remains limited. In this study, we show that the heterochromatin mark, H3K23me3, is induced by nuclear RNAi at both exogenous and endogenous targets in . In addition, dsRNA-induced H3K23me3 can persist for multiple generations after the dsRNA exposure has stopped. We demonstrate that the histone methyltransferase SET-32, methylates H3K23 . Both and the germline nuclear RNAi Argonaute, are required for nuclear RNAi-induced H3K23me3 . Our data poise H3K23me3 as an additional chromatin modification in the nuclear RNAi pathway and provides the field with a new target for uncovering the role of heterochromatin in transgenerational epigenetic silencing.
核 RNAi 为研究 RNA 介导的染色质变化和表观遗传遗传提供了一个高度可行的系统。最近的研究表明，核 RNAi 介导的异染色质的调节和功能非常复杂。我们对组蛋白修饰以及该系统中涉及的相应组蛋白修饰酶的了解仍然有限。在这项研究中，我们发现在外源和内源靶标中，核 RNAi 诱导了异染色质标记 H3K23me3。此外，dsRNA 诱导的 H3K23me3 在 dsRNA 暴露停止后可以持续多个世代。我们证明了组蛋白甲基转移酶 SET-32 使 H3K23 甲基化。和生殖系核 RNAi Argonaute 都是核 RNAi 诱导的 H3K23me3 所必需的。我们的数据使 H3K23me3 成为核 RNAi 途径中的另一种染色质修饰，并为揭示异染色质在跨代表观遗传沉默中的作用提供了一个新的研究靶点。
