School of Natural Sciences, Bangor University, UK.
Res Microbiol. 2020 Oct-Nov;171(7):215-221. doi: 10.1016/j.resmic.2020.07.003. Epub 2020 Aug 14.
A novel, obligately anaerobic, acidophilic bacterium (strain I2511), isolated from sediment in an abandoned copper mine, was shown to couple the oxidation of organic electron donors to the reduction of both zero-valent sulfur and ferric iron in acidic media. The isolate was an obligate heterotroph that used a variety of organic compounds as electron donors and required yeast extract for growth. Alternative electron acceptors (sulfate, tetrathionate, thiosulfate and nitrate) were not used by the novel isolate. The strain grew as motile, endospore-forming rods, and was mesophilic and moderately acidophilic, with a growth rate of 0.01 h at optimum pH (3.7) and temperature (35 °C). Analysis of its 16S rRNA gene sequence placed strain I2511 within the phylum Firmicutes, distantly related to validated species. Phylogenetic analysis and physiological traits indicate that the novel strain represents a species of a candidate novel genus. Strain I2511 was included in a microbial consortium in a low pH "hybrid" sulfidogenic bioreactor designed to remove chalcophilic metals from metal-contaminated liquors and was present in >50% relative abundance when bioreactor was operated at pH ∼ 2.0. Results indicate that the novel isolate could be applied in biotechnologies to treat acidic and neutral pH, metal-rich effluents.
从废弃铜矿中的沉积物中分离到一种新型的专性厌氧嗜酸细菌（菌株 I2511），该细菌能在酸性介质中将有机电子供体的氧化与零价硫和高铁的还原偶联。该分离株是一种专性异养生物，能利用多种有机化合物作为电子供体，并需要酵母提取物才能生长。新型分离株不能利用替代电子受体（硫酸盐、连四硫酸盐、硫代硫酸盐和硝酸盐）。该菌株以运动的、形成内生孢子的杆状形式生长，是中温嗜酸性的，在最适 pH（3.7）和温度（35°C）下的生长速率为 0.01 h。对其 16S rRNA 基因序列的分析将菌株 I2511 置于厚壁菌门内，与已验证的物种关系较远。系统发育分析和生理特性表明，新型菌株代表了候选新型属的一个种。菌株 I2511 被包含在一个微生物联合体中，该联合体位于一个低 pH 的“混合”硫化生物反应器中，用于从金属污染的液体中去除亲硫金属，当生物反应器在 pH 约为 2.0 时运行时，其相对丰度大于 50%。结果表明，新型分离株可应用于生物技术中，以处理酸性和中性 pH 值、富含金属的废水。
