TERT promoter mutations in primary and secondary WHO grade III meningioma.

PURPOSE

TERT promoter mutation (TERTp ) has a strong association to recurrence and has been suggested to act as a driver mutation for malignant transformation of WHO grade I and II meningiomas. TERTp has been investigated in selected high-grade meningioma samples. The existence of TERTp across recurrent tumors in a population-based cohort needs to be investigated in order to identify when TERTp emerges across recurrent samples and to validate prognostic impact among WHO grade III tumors.

METHODS

We gathered material from a consecutive single-center cohort of 40 patients with malignant meningioma (WHO grade III) treated between 2000 and 2018, including specimens from primary and secondary malignant meningiomas with the corresponding earlier benign specimens and later malignant recurrences. In total 107 tumor samples were studied by Sanger sequencing for TERT promoter mutational status.

RESULTS

Seven of 40 patients (17.5%) harbored TERTp thus validating the incidence of TERTp in previous non-population-based cohorts. In 6/7 patients, the TERTp was present at initial surgery (WHO grade I-III) while in one patient the TERTp was found de novo when the meningioma became malignant. The incidences were 2/1.000.000/year for TERTp WHO grade III meningioma and 8/1.000.000/year for TERTp WHO grade III meningioma in our catchment area. We found a 1.7 times higher recurrence rate (CI 95% 0.65-4.44) and a 2.5 higher mortality rate per 10 person-years (CI 95% 1.01-6.19) for TERTp compared to TERTp .

CONCLUSION

TERTp can occur independently of malignant progression in meningioma and was most often present from the first tumor sample across recurring tumors. TERTp in WHO grade III may represent a marker of an aggressive subset of tumors.