Key Laboratory of Regional Environment and Eco-restoration, Ministry of Education, Shenyang University, Shenyang 110044, China.
College of New Energy and Environment, Jilin University, Changchun 130021, China.
Molecules. 2020 Aug 12;25(16):3665. doi: 10.3390/molecules25163665.
This study focuses on the kinetics of a pure strain of bacterium sp. SKC, isolated from phenol-contaminated soil, for the biodegradation of phenol as its sole carbon and energy source in aqueous medium. The kinetics of phenol biodegradation including the lag phase, the maximum phenol degradation rate, maximum growth rate () and maximum yield coefficient (Y) for each (initial phenol concentration, mg/L) were fitted using the Gompertz and Haldane models of substrate inhibition (R > 0.9904, RMSE < 0.00925). The values of these parameters at optimum conditions were = 0.30 h, = 36.40 mg/L, and = 418.79 mg/L, and that means the inhibition concentration of phenol was 418.79 mg/L. By comparing with other strains of bacteria, sp. SKC exhibited a high yield factor and tolerance towards phenol. This study demonstrates the potential application of sp. SKC for the bioremediation of phenol contaminate.
本研究以从受酚污染土壤中分离出的纯菌株 sp. SKC 为研究对象，研究其在水相介质中以酚为唯一碳源和能源进行生物降解的动力学特性。采用 Gompertz 和 Haldane 模型对包括迟滞期、最大酚降解速率、最大生长速率 () 和最大产率系数 (Y) 在内的酚生物降解动力学进行拟合（R > 0.9904，RMSE < 0.00925）。在最佳条件下，这些参数的值分别为 = 0.30 h、 = 36.40 mg/L 和 = 418.79 mg/L，这意味着酚的抑制浓度为 418.79 mg/L。与其他细菌菌株相比，sp. SKC 表现出较高的产率因子和对酚的耐受性。本研究证明了 sp. SKC 在生物修复酚污染方面的潜在应用。
