Egozarian Natalia S, Emelyanova Elena V, Suzina Nataliya E, Sazonova Olesya I, Polivtseva Valentina N, Anokhina Tatiana O, Wu Yonghong, Solyanikova Inna P
G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Prospect Nauki 5, Pushchino 142290, Russia.
State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, 71 East Beijing Road, Nanjing 210008, China.
Microorganisms. 2024 Mar 16;12(3):597. doi: 10.3390/microorganisms12030597.
Biodegradation of phenol is an effective method for removing this toxicant from contaminated sites. Phenol is a toxic compound for living cells, so many bacteria degrade phenol in relatively low concentrations, up to 0.75 g L. The strain 1CP is an effective destructor of a wide range of pollutants. In the absence of a carbon source in the medium, cells of the 1CP strain easily form cyst-like resting cells (CLC). The purpose of this work was to evaluate the viability of cells during long-term storage and the efficiency of the process of phenol destruction by 1CP cells germinating after dormancy. Resting cells were obtained by simple cultivation in a rich medium followed by storage under static conditions. This is a simple approach to obtain a large amount of biomass. Decomposition of phenol proceeded via catechol followed by -cleavage of aromatic ring. The induction of three phenol hydroxylases was detected by RT-PCR in cells germinated in a mineral medium with phenol as the carbon source. The stability of the genome of cells germinating after dormancy is shown by box-PCR. Dormant 1CP cells, both suspended and immobilized, can be directly used for the decomposition of phenol after 4-12 months storage. In addition to phenol, after 9 months of storage, immobilized germinating cells easily metabolized 4-chlorophenol and 2,4,6-trichlorophenol. The results demonstrate a potential and simple approach toward achieving long-term storage of cells for further use in bioremediation.
苯酚的生物降解是从受污染场地去除这种有毒物质的有效方法。苯酚是一种对活细胞有毒的化合物，因此许多细菌能在相对低浓度（高达0.75 g/L）下降解苯酚。1CP菌株是多种污染物的有效分解者。在培养基中缺乏碳源的情况下，1CP菌株的细胞很容易形成囊状静止细胞（CLC）。这项工作的目的是评估长期储存期间细胞的活力以及休眠后萌发的1CP细胞对苯酚的破坏效率。通过在丰富培养基中简单培养，然后在静态条件下储存来获得静止细胞。这是一种获得大量生物质的简单方法。苯酚的分解通过邻苯二酚进行，随后芳香环发生β-裂解。通过RT-PCR在以苯酚作为碳源的矿物培养基中萌发的细胞中检测到三种苯酚羟化酶的诱导。通过盒式PCR显示了休眠后萌发细胞基因组的稳定性。休眠的1CP细胞，无论是悬浮的还是固定化的，在储存4 - 12个月后都可直接用于苯酚的分解。除了苯酚，储存9个月后，固定化的萌发细胞很容易代谢4-氯苯酚和2,4,6-三氯苯酚。结果证明了一种实现细胞长期储存以用于进一步生物修复的潜在且简单的方法。
