Department of Life and Food Sciences, Obihiro University of Agriculture and Veterinary Medicine, West 2-11, Inada, Obihiro 080-8555, Hokkaido, Japan.
Research Center for Global Agromedicine, Obihiro University of Agriculture and Veterinary Medicine, West 2-11, Inada, Obihiro 080-8555, Hokkaido, Japan.
Nutrients. 2020 Aug 12;12(8):2412. doi: 10.3390/nu12082412.
As raw sorghum is not able to influence considerable colonic fermentation despite its higher resistant starch (RS) content, our study aimed to investigate the effects of frozen autoclaved sorghum on colonic fermentation. Fischer 344 rats were fed frozen cooked refined (S-Rf) and whole (S-Wh) sorghum diets and were compared against α-corn starch (CON) and high amylose starch (HAS) fed rats for zoometric parameters, cecal biochemical and microbiological parameters. Sorghum fed rats exhibited significantly lower feed intake and visceral adipose tissue mass compared to CON. Bacterial alpha diversity was significantly higher in the sorghum fed rats compared to HAS and the two sorghum fed groups clustered together, separately from HAS and CON in the beta diversity plot. Serum non-High Density Lipoprotein cholesterol and total cholesterol in S-Rf group were significantly lower compared to CON, while total fecal bile excretion was also significantly higher in the two sorghum fed groups. Lower visceral adiposity was correlated with lower feed intake, RS content ingested and cecal short chain fatty acid (SCFA) contents. Thus, higher RS inflow to the colon via frozen autoclaved sorghum might have influenced colonic fermentation of RS and the resultant SCFA might have influenced lower adiposity as manifested by the lower body weight gain.
由于生高粱尽管其抗性淀粉（RS）含量较高，但不能对结肠发酵产生显著影响，因此我们的研究旨在探究冷冻高压灭菌高粱对结肠发酵的影响。研究人员将 Fischer 344 大鼠分别喂食冷冻熟化（S-Rf）和全粒（S-Wh）高粱饮食，并与喂食α-玉米淀粉（CON）和高直链淀粉（HAS）的大鼠进行比较，以评估其体型参数、盲肠生化和微生物学参数。与 CON 相比，喂食高粱的大鼠的饲料摄入量和内脏脂肪组织质量显著降低。与 HAS 相比，喂食高粱的大鼠的细菌 alpha 多样性显著更高，并且在 beta 多样性图谱中，这两个高粱喂养组与 HAS 和 CON 聚类在一起，彼此分开。S-Rf 组的血清非高密度脂蛋白胆固醇和总胆固醇水平明显低于 CON，而两组高粱喂养组的总粪便胆汁排泄量也明显更高。较低的内脏脂肪含量与较低的饲料摄入量、摄入的 RS 含量和盲肠短链脂肪酸（SCFA）含量相关。因此，通过冷冻高压灭菌高粱向结肠输入更多的 RS，可能会影响 RS 的结肠发酵，由此产生的 SCFA 可能会通过降低体重增加来影响较低的脂肪堆积。
