Al-Sadik Heba, Sugathan Subi, Saseedharan Prashanth, Sulaiman Shahrazad, Beegam Sumaya, Nemmar Abderrahim, Attoub Samir, Karam Sherif M
Department of Anatomy, College of Medicine & Health Sciences, United Arab Emirates University, P.O. Box 17666, Al-Ain, UAE.
Department of Pharmacology and Therapeutics, College of Medicine & Health Sciences, United Arab Emirates University, P.O. Box 17666, Al-Ain, UAE.
Life (Basel). 2020 Aug 12;10(8):149. doi: 10.3390/life10080149.
Stem cells have attracted many scientists because of their unique properties and therapeutic applications. However, very little is known on the environmental toxins that could affect their biological features. This study focuses on the consequences of the exposure of a cell line representative of the mouse gastric stem/progenitor (mGS) cells to diesel exhaust particles (DEPs). These immortal cells were cultured using routine protocols. The DEPs were added to the culture media at 1, 10, and 100 µg/mL for 1 to 72 h. The cells were assayed for their viability, migration, oxidative stress, and the expression of genes specific for cell proliferation, pluripotency, and death. DEPs induced a reduction in the metabolic activity of mGS cells, only at a high concentration of 100 µg/mL. However, no significant effects were detected on cell migration, oxidative stress markers (glutathione and thiobarbituric acid reactive substances), and cell death related proteins/genes. Interestingly, these findings were associated with down-regulation of Notch 2 and 3 and Bmi-1 proteins and activation of STAT3 involved in the regulation of the fate of stem cells. In conclusion, this study demonstrates that mGS cells have some resistance to oxidative stress and apoptosis when exposed to DEPs at the expense of their stemness.
干细胞因其独特的特性和治疗应用吸引了众多科学家。然而，对于可能影响其生物学特性的环境毒素，我们却知之甚少。本研究聚焦于将一种代表小鼠胃干细胞/祖细胞（mGS）的细胞系暴露于柴油尾气颗粒（DEP）的后果。这些永生化细胞采用常规方案进行培养。将DEP以1、10和100μg/mL的浓度添加到培养基中，作用1至72小时。对细胞进行活力、迁移、氧化应激以及细胞增殖、多能性和死亡相关基因表达的检测。仅在100μg/mL的高浓度下，DEP诱导mGS细胞的代谢活性降低。然而，在细胞迁移、氧化应激标志物（谷胱甘肽和硫代巴比妥酸反应性物质）以及细胞死亡相关蛋白/基因方面未检测到显著影响。有趣的是，这些发现与Notch 2和3以及Bmi-1蛋白的下调以及参与干细胞命运调控的STAT3的激活有关。总之，本研究表明，mGS细胞在暴露于DEP时对氧化应激和凋亡具有一定抗性，但以牺牲其干性为代价。
