Department of Pathology, The Ohio State University, Columbus, OH, USA.
Methods Mol Biol. 2021;2193:13-21. doi: 10.1007/978-1-0716-0845-6_2.
The ideal response to skin injury is the complete regeneration of normal tissue without scar formation. This regenerative response is known to occur at early stages of embryonic development but is lost as the skin becomes more mature. In more developed skin, the wound-healing response is suboptimal and results in the formation of scar tissue. Scar tissue can be a significant clinical concern, causing skin dysfunction as well as psychosocial issues related to poor aesthetic outcomes. Mouse models of fetal wound healing can be useful for understanding what regulatory pathways lead to skin regeneration and scarless healing in less developed skin or scarring and fibrotic healing in more developed skin. Here, a reproducible incisional wound model in developing mice is described that our lab has used repeatedly to study scarless and fibrotic fetal wound healing.
理想的皮肤损伤反应是正常组织的完全再生而无瘢痕形成。这种再生反应在胚胎发育的早期阶段发生，但随着皮肤的成熟而丧失。在更成熟的皮肤中，伤口愈合反应不理想，导致瘢痕组织形成。瘢痕组织可能是一个严重的临床问题，导致皮肤功能障碍以及与不良美学结果相关的心理社会问题。胎儿伤口愈合的小鼠模型可用于了解哪些调节途径导致欠发达皮肤的皮肤再生和无瘢痕愈合，或更发达皮肤的瘢痕和纤维化愈合。在这里，描述了一种在发育中的小鼠中可重复使用的、可复制的切口性伤口模型，我们实验室已反复使用该模型来研究无瘢痕和纤维化的胎儿伤口愈合。
