Department of Pharmaceutical Sciences, Jerry H. Hodge School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, TX, USA.
Methods Mol Biol. 2021;2193:23-30. doi: 10.1007/978-1-0716-0845-6_3.
Chronic nonhealing wounds impact nearly 15% of Medicare beneficiaries (8.2 million) in the United States costing $28-$32 billion annually. Despite advancement in wound management, approximately 8% of diabetic Medicare beneficiaries have a foot ulcer and 1.8% will have an amputation. The development of a regenerative approach is warranted to save these before-mentioned amputations. To this extent, herein, we describe the detailed methods in generating a type 1 diabetes mellitus (T1DM) condition in immunocompromised mice, inducing cutaneous wound, and application of dental pulp stem cell-derived secretory products for therapeutic assessment. This model helps in evaluating the efficacy of stem cell-based therapy and helps with the investigation of involved mechanisms in impaired cutaneous wound healing caused by hyperglycemic stress due to type 1 diabetes.
慢性难愈性伤口影响了美国近 15%的医疗保险受益人（820 万人），每年花费 280 亿至 320 亿美元。尽管伤口管理取得了进展，但大约 8%的糖尿病医疗保险受益人有足部溃疡，1.8%的人将进行截肢。为了避免这些截肢，有必要采用再生方法。在这方面，我们在此描述了在免疫功能低下的小鼠中产生 1 型糖尿病（T1DM）的详细方法，诱导皮肤伤口，并应用牙髓干细胞衍生的分泌产物进行治疗评估。该模型有助于评估基于干细胞的治疗效果，并有助于研究由于 1 型糖尿病引起的高血糖应激导致的受损皮肤伤口愈合的相关机制。
