颅脑创伤挫伤鼠模型：皮质撞击法。

Contusion Rodent Model of Traumatic Brain Injury: Controlled Cortical Impact.

机构信息

CNRS UMR5287, INCIA, University of Bordeaux, Bordeaux, France.

Institute for Stroke and Dementia Research (ISD), University of Munich Medical Center, Munich, Germany.

出版信息

Methods Mol Biol. 2021;2193:49-65. doi: 10.1007/978-1-0716-0845-6_6.

DOI:10.1007/978-1-0716-0845-6_6

PMID:32808258

Abstract

Traumatic brain injury (TBI) is a heterogeneous brain injury which represents one of the leading causes of mortality and disability worldwide. Rodent TBI models are helpful to examine the cellular and molecular mechanisms after injury. Controlled cortical impact (CCI) is one of the most commonly used TBI models in rats and mice, based on its consistency of injury and ease of implementation. Here, we describe a CCI protocol to induce a moderate contusion to the somatosensory motor cortex. We provide additional protocols for monitoring animals after CCI induction.

摘要

创伤性脑损伤（TBI）是一种异质性脑损伤，是全球范围内导致死亡和残疾的主要原因之一。啮齿动物 TBI 模型有助于研究损伤后的细胞和分子机制。基于其损伤的一致性和易于实施，控制性皮质撞击（CCI）是大鼠和小鼠中最常用的 TBI 模型之一。在这里，我们描述了一种用于诱导感觉运动皮层中度挫伤的 CCI 方案。我们还提供了 CCI 诱导后监测动物的附加方案。