Wei Xunyu, Yang Bing, Chen Guangjing, Wang Di, Shi Yue, Chen Qiaoli, Kan Jianquan
College of Food Science, Southwest University, Beibei, Chongqing, PR China.
Laboratory of Quality & Safety Risk Assessment for Agro-Products on Storage and Preservation (Chongqing), Ministry of Agriculture, Chongqing, PR China.
J Food Biochem. 2020 Oct;44(10):e13441. doi: 10.1111/jfbc.13441. Epub 2020 Aug 18.
To investigate the effect of Zanthoxylum alkylamides (ZA) on amino acid metabolism of type 2 diabetes mellitus (T2DM), the present study was performed with T2DM rats model induced with high fat and sugar fodder combined with low-dose of streptozotocin. ZA were fed to rats at three different doses of 2, 4, and 8 mg/kg for 28 days and metformin was fed to rats at 135 mg/kg as positive control. The results showed that compared with the normal control, the amino acid levels and the expression of related carrier genes were disordered in T2DM rats. Compared with the model, different doses of ZA could significantly resist (p < .05) the decrease in body weight of T2DM rats and improve hyperglycemia, with the best result observed with the high dose (8 mg/kg). Different doses of ZA could ameliorate the levels of 19 kinds of amino acid in the plasma, jejunum, liver, and skeletal muscle of T2DM rats by regulating the expression of related amino acid transporters including LAT1, SNAT2, CAT1, et al. to thereby ameliorating amino acid metabolism disorder in T2DM rats. PRACTICAL APPLICATIONS: Previous studies showed that Zanthoxylum alkylamides (ZA) could promote the amino acid metabolism in the jejunum of healthy SD rats, improve protein metabolism disorder of type 1 diabetic rats, and also reduce the risk of metabolic syndrome in fat rats model. Herein, we investigated the effect of ZA on amino acid metabolism in type 2 diabetes mellitus (T2DM) rats. The results indicated that ZA could remarkably improve the abnormal expression of amino acid carriers in the jejunum, liver, and skeletal muscle, thereby ameliorating the disorder of amino acid metabolism in the plasma, jejunum, liver, and skeletal muscle of T2DM rats. Therefore, ZA are potential antidiabetic food/medicine product for the T2DM treatment.
为研究花椒酰胺（ZA）对2型糖尿病（T2DM）氨基酸代谢的影响，本研究采用高脂高糖饲料联合低剂量链脲佐菌素诱导的T2DM大鼠模型进行。将ZA以2、4和8 mg/kg三种不同剂量喂给大鼠，持续28天，并将135 mg/kg的二甲双胍喂给大鼠作为阳性对照。结果显示，与正常对照组相比，T2DM大鼠的氨基酸水平和相关载体基因的表达紊乱。与模型组相比，不同剂量的ZA可显著抵抗（p < 0.05）T2DM大鼠体重的下降并改善高血糖，高剂量（8 mg/kg）效果最佳。不同剂量的ZA可通过调节包括LAT1、SNAT2、CAT1等相关氨基酸转运体的表达，改善T2DM大鼠血浆、空肠、肝脏和骨骼肌中19种氨基酸的水平，从而改善T2DM大鼠的氨基酸代谢紊乱。实际应用：先前的研究表明，花椒酰胺（ZA）可促进健康SD大鼠空肠中的氨基酸代谢，改善1型糖尿病大鼠的蛋白质代谢紊乱，还可降低肥胖大鼠模型中代谢综合征的风险。在此，我们研究了ZA对2型糖尿病（T2DM）大鼠氨基酸代谢的影响。结果表明，ZA可显著改善空肠、肝脏和骨骼肌中氨基酸载体的异常表达，从而改善T2DM大鼠血浆、空肠、肝脏和骨骼肌中的氨基酸代谢紊乱。因此，ZA是治疗T2DM的潜在抗糖尿病食品/药品。
