Department of Psychology and Neuroscience, Dalhousie University, Halifax, Nova Scotia, Canada.
Department of Psychiatry, Dalhousie University, Halifax, Nova Scotia, Canada.
Genes Brain Behav. 2021 Mar;20(3):e12696. doi: 10.1111/gbb.12696. Epub 2020 Sep 11.
The membrane-associated mucin (MAM) domain containing glycosylphosphatidylinositol anchor 2 protein single knock-out mice (MDGA2 ) are models of ASD. We examined the behavioral phenotypes of male and female MDGA2 and wildtype mice on C57BL6/NJ and C57BL6/N backgrounds at 2 months of age and measured MDGA2, neuroligin 1 and neuroligin 2 levels at 7 months. Mice on the C57BL6/NJ background performed better than those on the C57BL6/N background in visual ability and in learning and memory performance in the Morris water maze and differed in measures of motor behavior and anxiety. Mice with the MDGA2 genotype differed from WT mice in motor, social and repetitive behavior and anxiety, but most of these effects involved interactions between MDGA2 genotype and background strain. The background strain also influenced MDGA2 levels and NLGN2 association in MDGA2 mice. Our findings emphasize the importance of the background strain used in studies of genetically modified mice.
膜相关粘蛋白（MAM）结构域包含糖基磷脂酰肌醇锚定 2 蛋白单敲除小鼠（MDGA2）是 ASD 的模型。我们在 2 个月大时，在 C57BL6/NJ 和 C57BL6/N 背景下检查了雄性和雌性 MDGA2 及野生型小鼠的行为表型，并在 7 个月时测量了 MDGA2、神经连接蛋白 1 和神经连接蛋白 2 的水平。C57BL6/NJ 背景下的小鼠在视觉能力、莫里斯水迷宫中的学习和记忆表现以及运动行为和焦虑测量方面优于 C57BL6/N 背景下的小鼠。MDGA2 基因型的小鼠与 WT 小鼠在运动、社交和重复行为以及焦虑方面存在差异，但这些影响大多数涉及 MDGA2 基因型和背景品系之间的相互作用。背景品系也影响 MDGA2 小鼠中的 MDGA2 水平和 NLGN2 关联。我们的研究结果强调了在研究基因修饰小鼠时使用背景品系的重要性。
