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环境优化在储存公牛精子细胞中的作用。

The role of environmental optimization for storing bulls' sperm cells.

机构信息

Department of Environment, Animal Hygiene, and Welfare, Wrocław University of Environmental and Life Sciences , Wrocław, Poland.

Institute of Animal Breeding, Wrocław University of Environmental and Life Sciences , Wrocław, Poland.

出版信息

Syst Biol Reprod Med. 2020 Oct;66(5):300-310. doi: 10.1080/19396368.2020.1795432. Epub 2020 Aug 18.

Abstract

Artificial insemination has achieved a dynamic increase in genetic progress, and this is due to the improvement of sperm preservation technology. In recent years, a lot of attention has been paid to optimizing bull sperm storage environment and objectifying methods of sperm quality analysis. This review presents bull sperm preservation methods and ways to modify their storage environment. The main purpose of sperm preparation for artificial insemination is to obtain sperm with a high percentage of viable, motile sperm with normal morphology and low DNA fragmentation rates. Currently conducted experiments indicate the possibility of improving the quality of insemination doses produced using various components enriching common diluents. However, despite extensive research, no better results have been achieved than obtaining insemination doses with sperm viability that exceeds just over 60%. Obtaining a very good quality of frozen semen seems to be still unachievable today.

摘要

人工授精在遗传进展方面取得了显著的增长,这主要得益于精子保存技术的不断完善。近年来,人们越来越关注优化公牛精子储存环境和客观化精子质量分析方法。本综述介绍了公牛精子保存方法和改良其储存环境的途径。人工授精中精子制备的主要目的是获得具有高比例活率、运动正常形态和低 DNA 碎片率的精子。目前进行的实验表明,使用各种成分丰富普通稀释液来改善授精剂量的质量是可行的。然而,尽管进行了广泛的研究,目前还没有取得比精子活率超过 60%的授精剂量更好的效果。获得非常高质量的冷冻精液似乎仍然是遥不可及的。

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