基于 MetClo 的层级模块化 DNA 组装

Hierarchical Modular DNA Assembly Using MetClo.

机构信息

Nuffield Department of Medicine, Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK.

出版信息

Methods Mol Biol. 2020;2205:143-159. doi: 10.1007/978-1-0716-0908-8_9.

Abstract

DNA assembly methods are essential for multiple applications including synthetic biology. We recently developed MetClo, a method that uses a single type IIS restriction enzyme for hierarchical modular DNA assembly. This offers great flexibility in the design of the assembly experiment and simplicity of execution. Here we describe a protocol for hierarchical assembly of large DNA constructs from modular DNA parts using the MetClo vector set, a set of assembly vectors designed for the MetClo method.

摘要

DNA 组装方法在包括合成生物学在内的多个应用中至关重要。我们最近开发了 MetClo 方法，该方法使用单一类型 IIS 限制酶进行层次模块化 DNA 组装。这为组装实验的设计提供了极大的灵活性和执行的简单性。在这里，我们描述了使用 MetClo 载体集（一组专为 MetClo 方法设计的组装载体）从模块化 DNA 元件进行大型 DNA 构建体的层次组装的方案。