Biology Department, College of Science, University of Jeddah, Jeddah, Saudi Arabia.
Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
Acta Parasitol. 2021 Mar;66(1):136-145. doi: 10.1007/s11686-020-00267-2. Epub 2020 Aug 20.
Schistosomiasis is an acute and chronic disease of the genus Schistosoma triggered by blood flukes. Schistosomiasis is a disease occurring in, or endemic to, tropical and subtropical regions. A new concept was implemented to deal with schistosomiasis from natural plant sources. Curcumin's common name is Turmeric. Curcumin has proven to be main active component in Curcuma longa L. and has a wide range of anti-phrastic effects. Previous studies have shown the role of bone marrow mesenchymal stem cells (BMSCs) therapy in hepatic fibrosis recovery.
The current study was, therefore, intended to examine therapeutic role of BMSCs and Turmeric in murine schistosomiasis mansoni.
Mice were divided into five groups: a negative control group (non-infected non-treated), a positive control group (infected non-treated), a BMSCs treated group; Turmeric treated group, and untreated group. BMSCs derived from male mice were injected intraperitoneally into female mice receiving S. mansoni cercariae through the subcutaneous route. Liver histopathology and immuno-histochemical examinations were evaluated.
BMSCs intraperitoneal injection resulted in a significant reduction of liver collagen, granuloma size, and significant increase of OV-6 expression in the Schistosomiasis-treated mice group. There was overall improvement in pathological changes of the liver. Unfortunately, group IV showed a mild improvement in the granuloma size and fibrosis compared to corresponding BMSCs treatment group, although with vacuolated liver cells.
BMSCs have a regenerative potential in liver tissue histopathology by decreasing liver fibrosis and granulomas. Turmeric, by contrast, could not be used as an anti-fibrotic, according to the findings.
血吸虫病是一种由血吸虫子引发的急性和慢性疾病。血吸虫病是一种发生在热带和亚热带地区的疾病或地方性疾病。人们提出了一种从天然植物来源治疗血吸虫病的新概念。姜黄素的通用名是 turmeric。姜黄素已被证明是姜黄中的主要活性成分，具有广泛的抗药性作用。先前的研究表明骨髓间充质干细胞（BMSCs）治疗在肝纤维化恢复中的作用。
本研究旨在探讨 BMSCs 和 turmeric 在曼氏血吸虫病小鼠模型中的治疗作用。
将小鼠分为五组：阴性对照组（未感染未治疗）、阳性对照组（感染未治疗）、BMSCs 治疗组、turmeric 治疗组和未治疗组。从雄性小鼠中提取 BMSCs，通过皮下途径向接受曼氏血吸虫尾蚴的雌性小鼠腹腔内注射。评估肝组织病理学和免疫组织化学检查结果。
BMSCs 腹腔注射可显著减少血吸虫病治疗小鼠组的肝胶原、肉芽肿大小，并显著增加 OV-6 的表达。肝脏病理变化得到整体改善。不幸的是，与相应的 BMSCs 治疗组相比，第 IV 组的肉芽肿大小和纤维化仅略有改善，尽管肝细胞有空泡化。
BMSCs 通过减少肝纤维化和肉芽肿具有肝组织病理学再生潜力。然而，根据研究结果，turmeric 不能用作抗纤维化药物。
