Institute of Genetics and Molecular and Cellular Biology (IGBMC), Illkirch, France.
CNRS UMR7104, Illkirch, France.
Methods Mol Biol. 2021;2157:19-34. doi: 10.1007/978-1-0716-0664-3_3.
Chromosome conformation capture and its variants have allowed chromatin topology to be interrogated at a superior resolution and throughput than by microscopic methods. Among the method derivatives, 4C-seq (circular chromosome conformation capture, coupled to high-throughput sequencing) is a versatile, cost-effective means of assessing all chromatin interactions with a specific genomic region of interest, making it particularly suitable for interrogating chromatin looping events. We present the principles and procedures for designing and implementing successful 4C-seq experiments.
染色体构象捕获及其变体使得染色质拓扑结构的研究能够达到比显微镜方法更高的分辨率和通量。在这些方法的衍生物中，4C-seq（环状染色体构象捕获，与高通量测序相结合）是一种通用且具有成本效益的方法，可以评估与特定感兴趣基因组区域的所有染色质相互作用，因此特别适合研究染色质环化事件。我们介绍了设计和实施成功的 4C-seq 实验的原理和程序。
