College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510000, China.
State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China.
Fish Physiol Biochem. 2020 Dec;46(6):2133-2141. doi: 10.1007/s10695-020-00860-2. Epub 2020 Aug 22.
Basa catfish (Pangasius bocourti) is an indigenous species of the Lower Mekong River with increasing aquaculture value in Southeast Asia. Short-term semen storage has proven to be a valuable tool for assisted reproduction in fish, but little information is available on basa catfish. The present study was aimed at evaluating the effect of short-term semen storage on the sperm quality of basa catfish. Semen samples were kept at 4 °C for 7 days, either undiluted or diluted with Ca-F HBSS at different ratios (1:1, 1:3, and 1:6; semen:medium). Results showed that sperm quality was significantly affected by the time of chilled storage, characterized by a decline in sperm motility, viability, mitochondrial membrane potential (MMP), ATP content, and increased level of lipid peroxidation throughout the storage period. Compared with undiluted semen, diluted in Ca-F HBSS allowed better preservation of sperm quality during 7 days of chilled storage; dilution ratio at 1:1 was more effective than higher ratios (1:3 and 1:6) for prolonging sperm storability. In addition, sperm motility, viability, and ATP content decreased more rapidly than MMP, suggesting these indicators are more sensitive in detecting sperm damage of basa catfish during short-term chilled storage. These results obtained here will contribute to a better understanding of reproductive management in this species.
巴沙鱼（Pangasius bocourti）是湄公河流域的本地物种，在东南亚具有越来越高的水产养殖价值。短期精液储存已被证明是鱼类辅助繁殖的宝贵工具，但关于巴沙鱼的信息却很少。本研究旨在评估短期精液储存对巴沙鱼精子质量的影响。精液样本在 4°C 下储存 7 天，要么不稀释，要么用 Ca-F HBSS 以不同比例（1:1、1:3 和 1:6；精液：培养基）稀释。结果表明，冷藏储存时间对精子质量有显著影响，表现为精子活力、活力、线粒体膜电位（MMP）、ATP 含量下降，脂质过氧化水平在整个储存期间升高。与未稀释的精液相比，在 Ca-F HBSS 中稀释可在 7 天的冷藏储存期间更好地保持精子质量；与更高的稀释比例（1:3 和 1:6）相比，1:1 的稀释比例更有效地延长精子的储存能力。此外，精子活力、活力和 ATP 含量的下降速度快于 MMP，表明这些指标在检测巴沙鱼短期冷藏过程中的精子损伤时更为敏感。这些结果将有助于更好地了解该物种的生殖管理。
