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用 Th1/Th2 平衡佐剂配制融合前 RSV F 蛋白可在 RSV 经验丰富的年轻小鼠中提供完全保护，而不会产生 Th2 偏向性免疫。

Formulation of the prefusion RSV F protein with a Th1/Th2-balanced adjuvant provides complete protection without Th2-skewed immunity in RSV-experienced young mice.

机构信息

Department of Pharmacy and Therapeutics, University of Pittsburgh School of Pharmacy, Pittsburgh, PA, USA.

Department of Pharmacy and Therapeutics, University of Pittsburgh School of Pharmacy, Pittsburgh, PA, USA; Center for Clinical Pharmaceutical Sciences, University of Pittsburgh School of Pharmacy, Pittsburgh, PA, USA; Department of Medicine, Division of Internal Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

出版信息

Vaccine. 2020 Sep 22;38(41):6357-6362. doi: 10.1016/j.vaccine.2020.08.023. Epub 2020 Aug 20.

DOI:10.1016/j.vaccine.2020.08.023
PMID:32829976
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7540734/
Abstract

Respiratory syncytial virus (RSV) is a leading cause of lower respiratory tract infections among infants with most infections occurring in the first year of life. Multiple RSV exposures are required for children to mount adult-like immune responses. Although adult RSV immunity is associated with less severe disease, the protection induced through natural infection is short-lived. Therefore, vaccination of RSV-experienced young children may accelerate immunity and provide long-term protection from RSV reinfection. However, the extent to which different Th-biased vaccine regimens influence pre-existing humoral and cellular immunity in RSV-experienced young children is unknown. To address this question, infant BALB/c mice were RSV-infected and subsequently immunized with the prefusion RSV F (PreF) antigen formulated with either a Th2-skewing (Alum) or Th1/Th2-balanced (Advax-SM) adjuvant. These studies show that both adjuvants boosted neutralizing antibody and protected from RSV reinfection, but Advax-SM adjuvant prevented the Th2-skewed immunity observed in RSV-experienced young mice immunized with PreF/Alum.

摘要

呼吸道合胞病毒（RSV）是导致婴儿下呼吸道感染的主要原因，大多数感染发生在生命的第一年。儿童需要多次 RSV 暴露才能产生成人样的免疫反应。虽然成人 RSV 免疫力与疾病的严重程度较低相关，但通过自然感染诱导的保护是短暂的。因此，对 RSV 有经验的幼儿进行疫苗接种可能会加速免疫反应，并提供长期的 RSV 再感染保护。然而，不同的 Th 偏向疫苗方案在多大程度上影响 RSV 有经验的幼儿中已有的体液和细胞免疫尚不清楚。为了解决这个问题，用 RSV 感染了 RSV 经验丰富的婴儿 BALB/c 小鼠，然后用 Th2 偏向（铝佐剂）或 Th1/Th2 平衡（Advax-SM 佐剂）配制的前融合 RSV F（PreF）抗原进行免疫。这些研究表明，两种佐剂都能增强中和抗体并预防 RSV 再感染，但 Advax-SM 佐剂预防了用 PreF/Alum 免疫的 RSV 经验丰富的幼鼠中观察到的 Th2 偏向免疫。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c989/7540734/4c89f008f050/nihms-1621325-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c989/7540734/b65ece49b280/nihms-1621325-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c989/7540734/98b89dadb3d6/nihms-1621325-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c989/7540734/e5544202e918/nihms-1621325-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c989/7540734/4c89f008f050/nihms-1621325-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c989/7540734/b65ece49b280/nihms-1621325-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c989/7540734/98b89dadb3d6/nihms-1621325-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c989/7540734/e5544202e918/nihms-1621325-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c989/7540734/4c89f008f050/nihms-1621325-f0004.jpg

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本文引用的文献

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J Allergy Clin Immunol. 2019 Sep;144(3):796-808.e12. doi: 10.1016/j.jaci.2019.03.019. Epub 2019 Mar 30.
2
Humoral and cellular immunity to RSV in infants, children and adults.婴幼儿、儿童和成人对 RSV 的体液免疫和细胞免疫。
Vaccine. 2018 Oct 1;36(41):6183-6190. doi: 10.1016/j.vaccine.2018.08.056. Epub 2018 Aug 31.
3
Localization of the T-cell response to RSV infection is altered in infant mice.
工程化的 RSV 预融合 F 蛋白二酪氨酸键赋予了稳定性和效力优势。
Nat Commun. 2024 Mar 14;15(1):2202. doi: 10.1038/s41467-024-46295-8.
4
Ally, adversary, or arbitrator? The context-dependent role of eosinophils in vaccination for respiratory viruses and subsequent breakthrough infections.盟友、对手还是仲裁者？嗜酸性粒细胞在呼吸道病毒疫苗接种及其随后突破性感染中的上下文相关作用。
J Leukoc Biol. 2024 Jul 25;116(2):224-243. doi: 10.1093/jleuko/qiae010.
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Pediatr Pulmonol. 2018 Feb;53(2):145-153. doi: 10.1002/ppul.23911. Epub 2017 Nov 8.
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Adjuvants and the vaccine response to the DS-Cav1-stabilized fusion glycoprotein of respiratory syncytial virus.佐剂与针对呼吸道合胞病毒DS-Cav1稳定融合糖蛋白的疫苗反应
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J Immunol. 2005 Aug 1;175(3):1876-83. doi: 10.4049/jimmunol.175.3.1876.
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J Immunol. 2004 Feb 15;172(4):2549-58. doi: 10.4049/jimmunol.172.4.2549.
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Am J Respir Crit Care Med. 2003 Sep 15;168(6):633-9. doi: 10.1164/rccm.200210-1148OC. Epub 2003 May 28.