Laboratory of Marine Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Malaysia.
Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Malaysia.
J Fish Dis. 2020 Oct;43(10):1249-1258. doi: 10.1111/jfd.13222. Epub 2020 Aug 23.
Groupers are popular aquaculture species in South-East Asia, but their cultivation is affected by infectious disease outbreaks. Mucosa-associated lymphoid tissues provide a first-line defence against pathogens; however, few studies are available relating to cellular or proteomic responses of mucosal immunity in grouper. Skin, gill and intestine were sampled from brown-marbled grouper Epinephelus fuscoguttatus (Forsskål, 1775) at 4 and 96 hr post-infection (hpi) and 7 days post-infection (dpi) following intraperitoneal infection with Vibrio harveyi, and stained with haematoxylin/eosin and Alcian Blue/periodic acid-Schiff. Skin mucus was analysed by 2D-gel electrophoresis, and proteins modulated by the bacterial infection identified. In the infected fish, significant increases in sacciform cells in skin and increased levels of nucleoside diphosphate kinase in mucus were detected at 4 hpi. At 96 hpi, goblet cells containing acidic mucins significantly increased in the intestine, while those containing mixed mucins increased in skin and gills of infected fish. Proteasome subunit alpha type-I and extracellular Cu/Zn superoxide dismutase levels also increased in mucus. Rodlet and mast cells did not appear to respond to the infection. Mucosal tissues of grouper appeared actively involved in response to Vibrio infection. This information may help future research on improving grouper health, production and vaccine development.
斜带石斑鱼是东南亚地区一种受欢迎的水产养殖品种，但它们的养殖受到传染病爆发的影响。黏膜相关淋巴组织为病原体提供了第一道防线；然而，关于斜带石斑鱼黏膜免疫的细胞或蛋白质组学反应的研究很少。在腹腔内感染哈维弧菌后 4 和 96 小时以及 7 天，从褐点石斑鱼 Epinephelus fuscoguttatus（Forsskål，1775）中采集皮肤、鳃和肠组织，并用苏木精/伊红和阿尔辛蓝/过碘酸希夫染色。通过 2D 凝胶电泳分析皮肤黏液，并鉴定受细菌感染调节的蛋白质。在受感染的鱼中，在 4 小时时，皮肤中的囊泡细胞显著增加，黏液中的核苷二磷酸激酶水平升高。在 96 小时时，肠道中含有酸性粘蛋白的杯状细胞显著增加，而含有混合粘蛋白的杯状细胞在感染鱼的皮肤和鳃中增加。在黏液中，蛋白酶体亚单位 alpha 类型-I 和细胞外 Cu/Zn 超氧化物歧化酶水平也增加。棒状和肥大细胞似乎没有对感染产生反应。斜带石斑鱼的黏膜组织似乎积极参与了对弧菌感染的反应。这些信息可能有助于未来改善石斑鱼健康、生产和疫苗开发的研究。
