School of Chemistry, The University of Sydney, Sydney, NSW 2006, Australia.
School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.
Org Lett. 2020 Sep 4;22(17):6863-6867. doi: 10.1021/acs.orglett.0c02401. Epub 2020 Aug 24.
Interferon-γ (IFN-γ) is a glycoprotein that is responsible for orchestrating numerous critical immune induction and modulation processes and is used clinically for the treatment of a number of diseases. Herein, we describe the total chemical synthesis of homogeneously glycosylated variants of human IFN-γ using a tandem diselenide-selenoester ligation-deselenization strategy in the C- to N-terminal direction. The synthetic glycoproteins were successfully folded, and the structures and antiviral functions were assessed.
干扰素-γ（IFN-γ）是一种糖蛋白，负责协调许多关键的免疫诱导和调节过程，并在临床上用于治疗多种疾病。在此，我们描述了使用 C 到 N 端方向的串联二硒键-硒酯连接-去硒化策略对人 IFN-γ 的均一糖基化变体进行全化学合成。合成的糖蛋白成功折叠，并评估了其结构和抗病毒功能。
