Miyoshi Jun, Saito Daisuke, Nakamura Mio, Miura Miki, Mitsui Tatsuya, Kudo Toru, Murakami Shinnosuke, Matsuura Minoru, Hisamatsu Tadakazu
Department of Gastroenterology and Hepatology, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka-shi, Tokyo, Japan 1818611.
Nutrition Department, Kyorin University Hospital, 6-20-2 Shinkawa, Mitaka-shi, Tokyo, Japan 1818611.
Gastroenterol Res Pract. 2020 Aug 6;2020:7086939. doi: 10.1155/2020/7086939. eCollection 2020.
This prospective study included four healthy volunteers. The subjects continued their dietary habits for 2 weeks after the registration of the study and then started half-ED replacing 900 kcal of the regular diet with ED (time point 1, T1). The subjects continued half-ED for 2 weeks (T2). After the withdrawal of ED, subjects resumed their original dietary habits for 2 weeks (T3). Fecal samples were collected from all subjects at all time points, T1-3. Fecal DNA and metabolites were extracted from the samples. We performed 16S rRNA gene amplicon sequencing and metabolomic analysis to examine the bacterial compositions and intestinal metabolites.
There were differences in the gut bacterial compositions and metabolites at each time point as well as overtime changing patterns between subjects. Several bacteria and metabolites including short-chain fatty acids and bile acids altered significantly across the subjects. The bacterial membership and intestinal metabolites at T3 were different from T1 in all subjects.
Half-ED shifts the gut bacterial compositions and metabolites. The changes varied with each individual, while some microbes and metabolites change commonly across individuals. The impact of half-ED may persist even after the withdrawal. This trial is registered with UMIN ID: 000031920.
这项前瞻性研究纳入了四名健康志愿者。在研究登记后，受试者维持其饮食习惯两周，然后开始进行半能量替代饮食（half-ED），用能量替代饮食（ED）取代常规饮食中的900千卡（时间点1，T1）。受试者持续进行半能量替代饮食两周（T2）。在停用能量替代饮食后，受试者恢复其原来的饮食习惯两周（T3）。在所有时间点T1 - 3从所有受试者收集粪便样本。从样本中提取粪便DNA和代谢物。我们进行了16S rRNA基因扩增子测序和代谢组学分析，以检查细菌组成和肠道代谢物。
每个时间点以及受试者之间随时间的变化模式下，肠道细菌组成和代谢物都存在差异。包括短链脂肪酸和胆汁酸在内的几种细菌和代谢物在所有受试者中均有显著变化。所有受试者在T3时的细菌种类和肠道代谢物与T1时不同。
半能量替代饮食会改变肠道细菌组成和代谢物。变化因个体而异，而一些微生物和代谢物在个体间有共同变化。半能量替代饮食的影响即使在停用后可能仍会持续。本试验已在UMIN注册，编号：000031920。
