Byakika Stellah, Mukisa Ivan Muzira, Byaruhanga Yusuf Byenkya, Muyanja Charles
Department of Food Technology and Nutrition, School of Food Technology Nutrition and Bioengineering, College of Agricultural and Environmental Sciences, Makerere University, P O Box 7062, Kampala, Uganda.
Int J Microbiol. 2020 Aug 4;2020:7825943. doi: 10.1155/2020/7825943. eCollection 2020.
The purpose of this study was to establish the probiotic potential of lactic acid bacteria (LAB) starter cultures, MNC 21, MNC 24, and MNC 20, isolated from a traditionally fermented sorghum-millet beverage from Uganda. The cultures were examined for tolerance to acid and bile salts, bile salt hydrolase (BSH) activity, antibiotic susceptibility, biogenic amine production, mucin degradation, hydrophobicity, auto-aggregation, adherence to the ileum, coaggregation, and antimicrobial properties against selected pathogenic species. yoba 2012, a known probiotic, was the reference. The isolates were tolerant to acid (pH = 3) and bile (1%). . MNC 20 and . MNC 21 exhibited medium BSH activity (11-15 mm diameter of hydrolysis zone) while . and . yoba 2012 exhibited low BSH activity (<10 mm diameter of hydrolysis zone). All isolates lacked mucolytic activity. . MNC 21 and . MNC 20 produced agmatine. The candidate and reference microorganisms were resistant to 10 of 21 and 5 of 21 antibiotics, respectively. The isolates exhibited hydrophobic, auto-aggregation and coaggregation properties. These three properties were exhibited more ( < 0.05) by the reference than the potential probiotics. The ability of the potential probiotics to attach onto the goat ileum (7.3-8.0 log cfu/cm) was comparable to that of . yoba 2012 (7.6 log cfu/cm). The four LAB inhibited , , and to the same extent ( < 0.05). The findings indicated potential probiotic activity of the starter cultures. However, further examination of these isolates is required to confirm their probiotic capabilities.
本研究的目的是确定从乌干达一种传统发酵的高粱-小米饮料中分离出的乳酸菌（LAB）发酵剂培养物MNC 21、MNC 24和MNC 20的益生菌潜力。对这些培养物进行了耐酸和耐胆盐能力、胆盐水解酶（BSH）活性、抗生素敏感性、生物胺产生、黏蛋白降解、疏水性、自聚集、回肠黏附、共聚集以及对选定病原菌的抗菌特性检测。已知的益生菌约巴2012作为对照。这些分离株对酸（pH = 3）和胆汁（1%）具有耐受性。MNC 20和MNC 21表现出中等的BSH活性（水解圈直径为11 - 15毫米），而约巴2012表现出较低的BSH活性（水解圈直径<10毫米）。所有分离株均缺乏溶黏蛋白活性。MNC 21和MNC 20产生了胍丁胺。候选微生物和对照微生物分别对21种抗生素中的10种和5种具有抗性。这些分离株表现出疏水性、自聚集和共聚集特性。与潜在益生菌相比，对照在这三种特性上表现更明显（P<0.05）。潜在益生菌附着于山羊回肠的能力（7.3 - 8.0 log cfu/cm）与约巴2012（7.6 log cfu/cm）相当。这四种乳酸菌对大肠杆菌、金黄色葡萄球菌和白色念珠菌的抑制程度相同（P<0.05）。研究结果表明这些发酵剂培养物具有潜在的益生菌活性。然而，需要对这些分离株进行进一步检测以确认它们的益生菌能力。
