Delgadillo-Durán Paola, Soto-Suárez Mauricio, Rodriguez-Polanco Leonora, Carrero-Gutierrez Martha, Torres-Rojas Esperanza, Yockteng Roxana
Corporación Colombiana de Investigación Agropecuaria (AGROSAVIA), Centro de Investigación Tibaitatá, Km 14 vía Mosquera, Bogotá, Colombia.
Corporación Colombiana de Investigación Agropecuaria (AGROSAVIA), Centro de Investigación Nataima, Km 9 vía Espinal-Chicoral, Tolima, Colombia.
Plant Methods. 2020 Aug 19;16:114. doi: 10.1186/s13007-020-00656-8. eCollection 2020.
The black pod disease affects cacao plantations worldwide; it is caused by the oomycete species of the genus . The resistance of cacao plants to the black pod is commonly evaluated by artificial inoculation of the pathogen and the monitoring of the disease symptoms. However, it is difficult to identify resistant plants because the commonly used methods for the inoculation of the pathogens produce inconsistent results. Therefore, this study aimed to develop an efficient and reliable method to evaluate the resistance of seedlings to the infection by
Seedlings of different cacao genotypes were inoculated with under greenhouse conditions using the previously reported inoculation methods and a newly proposed method, the agar-water solution method. While none of the previously reported methods was effective, the agar-water solution method ensured a 100% seedling infection under greenhouse conditions. The proposed agar-water methodology is fast, simple and reproducible. Furthermore, the evaluation of this method in susceptible (CCN-51) and tolerant (SCA-6) genotypes produced the expected contrasting results.
The agar-water solution method presented in this study is an efficient alternative inoculation protocol for the identification of cacao genotypes that are resistant to black pod under greenhouse conditions.
黑荚病影响着全球的可可种植园；它由疫霉属的卵菌物种引起。可可植株对黑荚病的抗性通常通过人工接种病原体并监测病害症状来评估。然而，由于常用的病原体接种方法产生的结果不一致，难以鉴定出抗性植株。因此，本研究旨在开发一种高效且可靠的方法来评估可可幼苗对感染的抗性。
在温室条件下，使用先前报道的接种方法和新提出的琼脂 - 水溶液法，对不同可可基因型的幼苗接种病原体。虽然先前报道的方法均无效，但琼脂 - 水溶液法在温室条件下确保了100%的幼苗感染。所提出的琼脂 - 水方法快速、简单且可重复。此外，在易感（CCN - 51）和耐受（SCA - 6）基因型中对该方法的评估产生了预期的对比结果。
本研究中提出的琼脂 - 水溶液法是一种高效的替代接种方案，用于在温室条件下鉴定对黑荚病具有抗性的可可基因型。
