1st Department of Cardiology, Medical University of Gdansk, Gdansk, Poland.
Department of Clinical Chemistry, Medical University of Gdansk, Gdansk, Poland.
Oxid Med Cell Longev. 2020 Aug 5;2020:9709542. doi: 10.1155/2020/9709542. eCollection 2020.
Lipoprotein apheresis (LA) treatment results in a substantial reduction of low-density lipoprotein- (LDL-) cholesterol and lipoprotein(a) concentrations, which consequently decreases the rate of cardiovascular events. The additional benefit of LA may be associated with its impact on the composition and quality of high-density lipoprotein (HDL) particles, inflammation, and oxidative stress condition. To verify the effects of LA procedure, the current study is aimed at analyzing the effect of a single apheresis procedure with direct hemadsorption (DALI) and cascade filtration (MONET) on oxidative stress markers and HDL-related parameters. The study included eleven patients with familial hypercholesterolemia and hyperlipoproteinemia(a) treated with regular LA (DALI or MONET). We investigated the pre- and postapheresis concentration of the lipid-related oxidative stress markers 8-isoPGF2, oxLDL, TBARS, and PON-1. We also tracked potential changes in the main HDL apolipoproteins (ApoA-I, ApoA-II) and cholesterol contained in HDL subfractions. A single session of LA with DALI or MONET techniques resulted in a similar reduction of lipid-related oxidative stress markers. Concentrations of 8-isoPGF2 and TBARS were reduced by ~60% and ~30%, respectively. LA resulted in a 67% decrease in oxLDL levels along with a ~19% reduction in the oxLDL/ApoB ratio. Concentrations of HDL cholesterol, ApoA-I, ApoA-II, and PON-1 activity were also reduced by LA sessions, with more noticeable effects seen in the MONET technique. The quantitative proportions between HDL and HDL cholesterol did not change significantly by both methods. In conclusion, LA treatment with MONET or DALI system has a small nonselective effect on lowering HDL particles without any changes in the protein composition of these particles. Significant reduction in the level of oxidative stress parameters and less oxidation of LDL particles may provide an additional benefit of LA therapy.
脂蛋白吸附（LA）治疗可显著降低低密度脂蛋白（LDL）胆固醇和脂蛋白（a）浓度，从而降低心血管事件的发生率。LA 的额外益处可能与其对高密度脂蛋白（HDL）颗粒的组成和质量、炎症和氧化应激状态的影响有关。为了验证 LA 程序的效果，本研究旨在分析单次直接血液吸附（DALI）和级联过滤（MONET）LA 程序对氧化应激标志物和 HDL 相关参数的影响。该研究纳入了 11 名患有家族性高胆固醇血症和高脂蛋白（a）血症的患者，他们接受了常规 LA（DALI 或 MONET）治疗。我们研究了脂质相关氧化应激标志物 8-异前列腺素 F2（8-isoPGF2）、氧化型低密度脂蛋白（oxLDL）、TBARS 和 PON-1 在治疗前后的浓度。我们还跟踪了 HDL 载脂蛋白（ApoA-I、ApoA-II）和 HDL 亚组分中胆固醇的潜在变化。单次 DALI 或 MONET 技术的 LA 治疗导致脂质相关氧化应激标志物的降低程度相似。8-isoPGF2 和 TBARS 的浓度分别降低了约 60%和 30%。LA 使 oxLDL 水平降低了 67%，oxLDL/ApoB 比值降低了 19%。LA 还降低了 HDL 胆固醇、ApoA-I、ApoA-II 和 PON-1 活性的浓度，其中 MONET 技术的效果更为明显。两种方法均未使 HDL 与 HDL 胆固醇的定量比例发生显著变化。总之，MONET 或 DALI 系统的 LA 治疗对降低 HDL 颗粒具有较小的非选择性作用，而这些颗粒的蛋白质组成没有变化。氧化应激参数水平的显著降低和 LDL 颗粒的氧化程度降低可能为 LA 治疗提供额外的益处。
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