Szczepańska Katarzyna, Urbaniak Jacek, Śliwa Lucyna
Department of Botany and Plant Ecology, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland.
Department of Lichenology, W. Szafer Institute of Botany, Polish Academy of Sciences, Kraków, Poland.
PeerJ. 2020 Aug 3;8:e9555. doi: 10.7717/peerj.9555. eCollection 2020.
(Nyl.) R. Sant., a saxicolous, placodioid lichen, is considered to have a worldwide distribution in warm-temperate to boreal-arctic areas in Asia, Europe and North America. However, recent studies have revealed that this species includes five unrecognized species-level lineages-'subd A, B, C, D and E'. During research focused on the diversity of saxicolous lichens in mountainous areas of southern Poland, some interesting representatives of the genus were found. The main aim of our study was to determine the taxonomic status of the collected specimens by means of molecular tools and a comparative analysis of similar herbarium materials.
Detailed morphological, anatomical and chemical examinations of reference material from Asia, Europe and North and South America focused primarily on a selected group of lecanoroid taxa with a placodioid thallus. In addition, 21 new generated sequences representing s. lat and were selected for molecular study using the internal transcribed spacer region (ITS rDNA), together with 95 available GenBank sequences mainly from the genus
Polish specimens that clustered with members of a potential species-level lineage 'subd E' of complex were recovered. Comprehensive analyses of the lichen group led us to the conclusion that lineage 'subd E' represents s. str. and that the taxon appears to have a limited geographical distribution and specific habitat preferences. Furthermore, some of the recently defined species candidates within s. lat.-'subd D' and 'subd A'-should be assigned to two previously known species of , namely (Brodo) Leavitt, Zhao Xin & Lumbsch and (Poelt) Leavitt, Zhao Xin & Lumbsch, respectively. These two species are characterized by phenotypic features observed as well in analyzed specimens representing lineages 'subd D' and 'subd A'. Moreover, the representatives of these lineages demonstrate some differences in occupied habitat and geographical range that also correspond with the indicated species. Additionally, it was found that B.D. Ryan is a strongly supported monophyletic lineage within and therefore an appropriate new combination for the species is proposed.
（Nyl.）R. Sant. 是一种生于岩石上的叶状地衣，被认为在亚洲、欧洲和北美的暖温带至寒带北极地区广泛分布。然而，最近的研究表明，该物种包含五个未被认可的物种级谱系——“亚分支A、B、C、D和E”。在对波兰南部山区石生地衣多样性的研究中，发现了该属的一些有趣代表。我们研究的主要目的是通过分子工具和对类似标本馆材料的比较分析来确定所采集标本的分类地位。
对来自亚洲、欧洲以及北美洲和南美洲的参考材料进行详细的形态、解剖和化学检查，主要集中在一组具有叶状地衣体的茶渍型类群上。此外，选择了代表广义的该属和狭义的该属的21个新生成序列，用于使用内部转录间隔区（ITS rDNA）进行分子研究，同时还使用了95个主要来自该属的可用GenBank序列。
与该复合体潜在物种级谱系“亚分支E”成员聚类的波兰标本被发现。对地衣类群的综合分析使我们得出结论，谱系“亚分支E”代表狭义的该属，并且该分类单元似乎具有有限的地理分布和特定的栖息地偏好。此外，广义的该属中一些最近定义的候选物种——“亚分支D”和“亚分支A”——应分别归入该属的两个先前已知物种，即（Brodo）Leavitt、Zhao Xin & Lumbsch和（Poelt）Leavitt、Zhao Xin & Lumbsch。这两个物种的特征也在代表谱系“亚分支D”和“亚分支A”的分析标本中观察到。此外，这些谱系的代表在占据的栖息地和地理范围上表现出一些差异，这也与所指出的物种相对应。此外，发现B.D. Ryan是该属内一个得到有力支持的单系谱系，因此提出了该物种的一个合适新组合。
