Department of Mechanical Engineering, Chonnam National University, 77 Youngbong-ro, Buk-Gu, Gwangju 61186, Republic of Korea.
Lab Chip. 2020 Nov 24;20(23):4433-4441. doi: 10.1039/d0lc00501k.
A microfluidic circuit on a disk platform, also known as lab-on-a-disk, is an integrated system for automated high-throughput screening for biochemical analysis. The microfluidic circuit on a disk performs biochemical analysis through sequential processes such as filtration, separation, detection, and synthesis of reagents. Sequential processes in microfluidic circuits operate through the systematically linked components, which include channels, valves, and chambers. The microchannels should have micrometer-scale for precise micro-volume liquid control in the microfluidic circuit on a disk. However, it is difficult to also consider productivity in the traditional technology. In addition, as the channel length increases, much effort is required to construct the components of the microfluidic circuit in the limited space of the disk. 3D printing is drawing attention as a microfluidic channel fabrication technique in order to overcome the physical limitations of the traditional methods. A new concept of a 3D slope valve has been developed, which performs precise and sequential micro-volume liquid control through centrifugal and gravitational forces. Micro-volumes of liquids in a slope valve-equipped circuit are controlled over a wide range of angular velocities through the control of the valve geometry, types of liquid and volume. For sequential micro-volume of liquid control, three lines of assembled modules are connected to a microfluidic circuit. In the microfluidic circuit with slope valves, the detection of fluorescent dye tagged-VEGF is possible through sequential mixing and reaction processes. As a result, micro-volume liquid is successfully controlled with high accuracy using the 3D microfluidic circuit with a slope valve.
一种基于磁盘平台的微流控芯片，也称为芯片实验室，是一种用于生化分析自动化高通量筛选的集成系统。磁盘上的微流控芯片通过过滤、分离、检测和试剂合成等顺序过程进行生化分析。微流控芯片中的顺序过程通过系统连接的组件来操作，这些组件包括通道、阀门和腔室。微通道应该具有微米级尺度，以便在磁盘上的微流控芯片中精确控制微体积液体。然而，在传统技术中，同时考虑生产率是很困难的。此外，随着通道长度的增加，在磁盘的有限空间内构建微流控芯片的组件需要大量的工作。3D 打印作为一种微流道制造技术引起了人们的关注，以克服传统方法的物理限制。已经开发出一种新的 3D 斜坡阀的概念，它通过离心力和重力来实现精确和顺序的微体积液体控制。通过控制阀门几何形状、液体类型和体积，可以在宽角速度范围内控制配备斜坡阀的微流道中的微体积液体。对于顺序的微体积液体控制，三个组装模块的线连接到一个微流控芯片。在带有斜坡阀的微流控芯片中，可以通过顺序混合和反应过程来检测荧光标记的 VEGF。结果，使用带有斜坡阀的 3D 微流控芯片成功地以高精度控制微体积液体。
