Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
Org Biomol Chem. 2020 Nov 4;18(42):8620-8624. doi: 10.1039/d0ob00899k.
We developed a new class of turn-on fluorescent probes for an esterase. After the esterase-mediated hydrolysis produced carboxylate (as a fluorescence activator), the fluorescence intensity was markedly increased through the detachment of a quencher moiety from the quenched Cy5 fluorophore. Because the probes based on this new activator-induced quencher-detachment (AiQd) adopt a non-immolative linker between the cleavable site and the fluorophore, the rate of the enzymatic reaction is greatly improved, without the generation of any by-products.
我们开发了一类新型的酯酶荧光探针。在酯酶介导的水解产生羧酸根（作为荧光激活剂）后，荧光强度通过从猝灭的 Cy5 荧光团上脱离猝灭部分而显著增强。由于基于这种新的激活剂诱导的猝灭体脱离（AiQd）的探针在可切割位点和荧光团之间采用非消耗性连接体，因此酶反应的速率大大提高，而没有任何副产物的生成。
