Düzgün F, Dickgiesser N, Schuster D, Lengfelder E, Teuber J
Institut für Medizinische Mikrobiologie und Hygiene, Klinikum der Stadt Mannheim, Mannheim.
Klin Wochenschr. 1988 Mar 1;66(5):212-5. doi: 10.1007/BF01728199.
51 human sera containing antibodies to human immunodeficiency virus 1 (= HIV-1) were examined for HIV-1-antigen by three different enzyme immunoassay procedures (= EIA) of Abbott, Organon and Dupont. Sensibilities, handling as well as the correlation with the clinical stages of HIV-infection were compared. The EIA's diagnosed in accordance 6 sera which contained HIV-1-antigen and 42 sera to be HIV-1-antigen negative. 3 sera showed differences: according to the EIA of Organon none of these sera contained HIV-1-antigen, the EIA of Abbott (but not of Dupont) analysed HIV-1-antigen in one of these sera, in the other two sera only the EIA of Dupont showed HIV-1-Antigen. It is concluded that the differences in these 3 serum samples may originate not only in the different types of EIA used (indirect/direct procedure) but also in the different capture antibodies provided (antibodies against p-24 antigen or polyvalent antibodies).
采用雅培、欧加农和杜邦公司的三种不同酶免疫测定法（EIA），对51份含抗人类免疫缺陷病毒1型（=HIV-1）抗体的人血清进行HIV-1抗原检测。比较了这些方法的灵敏度、操作便利性以及与HIV感染临床阶段的相关性。酶免疫测定法诊断出6份血清含有HIV-1抗原，42份血清HIV-1抗原阴性。3份血清结果有差异：根据欧加农公司的酶免疫测定法，这些血清均不含HIV-1抗原；雅培公司的酶免疫测定法（杜邦公司的则未检测出）分析其中1份血清含有HIV-1抗原；另外2份血清中，只有杜邦公司的酶免疫测定法显示含有HIV-1抗原。得出结论：这3份血清样本结果的差异可能不仅源于所使用的不同类型酶免疫测定法（间接/直接法），还源于所提供的不同捕获抗体（抗p-24抗原抗体或多价抗体）。
