Griswold J, Maier R V
Department of Surgery, University of Washington, Seattle.
J Surg Res. 1988 Apr;44(4):417-24. doi: 10.1016/0022-4804(88)90184-9.
Depressed neutrophil (PMN) phagocytosis in patients with ARDS may contribute to the known increased incidence of pulmonary sepsis. To evaluate changes in phagocytosis, circulating PMNs from normal rats were compared to circulating and alveolar PMNs (obtained by bronchoalveolar lavage, BAL) from rats after 72 hr of endotoxin infusion (LPS-Rx)-induced acute lung injury. Since phagocytosis correlates with adherence, PMN adherence to coverslips and to a standard nylon wool column was also measured. PMN adherence to nylon wool was 65% for control, 77% for circulating LPS-Rx, and 20% for BAL PMNs. As a measure of phagocytosis the PMNs were incubated for 30 min with opsonized fluorescent (FITC) tagged yeast. Total PMN with yeast were 95.4 +/- 2.1% for control; 96.4 +/- 1.8% for circulating LPS-Rx; and 78.7 +/- 7.8% (P less than 0.05 compared to control) for BAL PMNs. Total numbers of yeast particles per 100 PMN are 270 +/- 64 for control, 300 +/- 42 for circulating LPS-Rx, and 170 +/- 45 (P less than 0.05 compared to control) for BAL PMN.
(1) Intraalveolar (BAL) PMNs have decreased adherence; (2) nonadherent PMNs have decreased uptake of yeast; (3) BAL PMNs, overall, have a significantly decreased uptake of yeast; (4) this depression in BAL PMN phagocytosis may partially explain the known decreased rate of bacterial clearance in injured lungs and the increased risk of pulmonary sepsis with adult respiratory distress syndrome.
急性呼吸窘迫综合征（ARDS）患者中性粒细胞（PMN）吞噬功能降低可能是导致已知肺部脓毒症发病率增加的原因。为了评估吞噬功能的变化，将正常大鼠的循环PMN与内毒素输注（LPS-Rx）诱导的急性肺损伤72小时后的大鼠循环和肺泡PMN（通过支气管肺泡灌洗获得，BAL）进行比较。由于吞噬作用与黏附相关，还测量了PMN对盖玻片和标准尼龙毛柱的黏附。对照组PMN对尼龙毛的黏附率为65%，循环LPS-Rx组为77%，BAL PMN组为20%。作为吞噬作用的指标，将PMN与调理过的荧光（FITC）标记酵母孵育30分钟。吞噬酵母的PMN总数在对照组为95.4±2.1%；循环LPS-Rx组为96.4±1.8%；BAL PMN组为78.7±7.8%（与对照组相比P<0.05）。每100个PMN中酵母颗粒的总数在对照组为270±64，循环LPS-Rx组为300±42，BAL PMN组为170±45（与对照组相比P<0.05）。
（1）肺泡内（BAL）PMN黏附降低；（2）非黏附性PMN对酵母的摄取减少；（3）总体而言，BAL PMN对酵母的摄取显著减少；（4）BAL PMN吞噬功能的这种降低可能部分解释了已知的损伤肺脏细菌清除率降低以及成人呼吸窘迫综合征患者肺部脓毒症风险增加的原因。
