Weber A, Smith A L, Wong K, Painter B, Krol G
Division of Infectious Disease, Children's Hospital and Medical Center, Seattle, WA.
Methods Find Exp Clin Pharmacol. 1988 Feb;10(2):123-7.
Two high pressure liquid chromatography methods developed independently and two different microbiological methods assayed samples containing ciprofloxacin derived from four volunteers: 46 sera, 19 saliva and 27 urine specimens. Each subject had received 500 mg of ciprofloxacin by mouth, with the samples collected in the ensuing six hours. Fifteen mock unknowns, prepared by adding known amounts of ciprofloxacin to normal human serum, were also assayed by the four procedures. The limits of sensitivity of the HPLC assays were less than 0.01 microgram/ml; the sensitivity limits of the bioassay were 0.02 microgram/ml (disc method) and 0.06 microgram/ml (well method). The inter-assay data was analyzed by linear regression. Ciprofloxacin concentrations in serum samples correlated well (R = 0.90) with all methods. Values obtained with urine samples also correlated well (R greater than or equal to 0.80). We conclude that either microbiological assay or quantitation by HPLC is satisfactory for quantitation of ciprofloxacin.
46份血清、19份唾液和27份尿液标本。每位受试者口服了500毫克环丙沙星，并在随后的6小时内采集样本。通过向正常人血清中添加已知量的环丙沙星制备的15份模拟未知样本也采用这四种方法进行了检测。高效液相色谱法的灵敏度极限小于0.01微克/毫升；生物测定法的灵敏度极限分别为0.02微克/毫升（纸片法）和0.06微克/毫升（孔板法）。通过线性回归分析了不同检测方法之间的数据。血清样本中环丙沙星浓度与所有方法的相关性都很好（R = 0.90）。尿液样本获得的值相关性也很好（R≥0.80）。我们得出结论，微生物学检测或高效液相色谱法定量对于环丙沙星的定量都是令人满意的。
