体外研究用于治疗髁突骨折的三种三维（3D）钛微型板系统对 THP-1 巨噬细胞中白细胞介素 1（IL-1）和白细胞介素 6（IL-6）浓度的影响。

In vitro effect of three-dimensional (3D) titanium mini-plate systems used for surgical treatment of condylar fractures on interleukin 1 (IL-1) and interleukin 6 (IL-6) concentration in THP-1 macrophages.

机构信息

Department of Maxillofacial Surgery, Hospital of the Ministry of Interior, Kielce, Wojska Polskiego 51, 25-375, Kielce, Poland; Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Powstańców Wlkp. 72, 70-111, Szczecin, Poland.

Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Powstańców Wlkp. 72, 70-111, Szczecin, Poland.

出版信息

Tissue Cell. 2020 Dec;67:101404. doi: 10.1016/j.tice.2020.101404. Epub 2020 Jul 6.

DOI:10.1016/j.tice.2020.101404

PMID:32835937

Abstract

About 20 %-35 % of mandibular fractures occur in the condylar process, a complication frequently associated with craniofacial traumas. Compared to other craniofacial fractures, some controversy remains around the effectiveness of the various treatment methods. It has been suggested that condylar osteosynthesis using mini-plates - a technique widely used by maxillofacial surgeons - may activate a pro-inflammatory response which is mediated by interleukins, later involved in bone remodelling and tissue regeneration. This study aimed at examining the influence of three-dimensional (3D) titanium mini-plate systems and the dedicated screws used in the surgical treatment of condylar fractions on the concentrations of interleukin 1(IL-1) and interleukin 6 (IL-6) in macrophages obtained from THP-1 monocytes. The cells were cultured for 24 h and 48 h with the 3D titanium condylar plates and dedicated screws (Synthes, Martin, Medartis manufacturer). The concentrations of IL-1 and IL-6 were measured using the ELISA method. Incubation of macrophages with plates did not cause a significant increase in IL-1 (for: Synthes 0.89-0.86 pg/mg protein; Martin 1.10-0.80 pg/mg protein; Medartis 1.20-0.84 pg/mg protein) and IL-6 (for Synthes 16.00-14.00 pg/mg protein, Martin 13.0-10.0 pg/mg protein; Medartis 9.0-12.0 pg/mg protein) expression for any of the plates used, compared to THP-1 macrophages incubated for 48 h under control conditions. Neither three-dimensional titanium mini-plates nor dedicated screws caused any changes in IL-1 and IL-6 expression in THP-1 macrophages, which is an important observation for clinicians treating condylar fractures. It confirms that titanium plates can be a safe/neutral material for humans, especially considering their significant influence on the osteoclast functions and bone remodelling processes after implantation.

摘要

约 20%-35%的下颌骨骨折发生在髁突，这是一种常与颅面创伤相关的并发症。与其他颅面骨折相比，各种治疗方法的有效性仍存在一些争议。有人认为，使用微型板进行髁突骨内固定——这是颌面外科医生广泛使用的一种技术——可能会激活由白细胞介素介导的促炎反应，随后参与骨重塑和组织再生。本研究旨在研究三维（3D）钛微型板系统和用于治疗髁突骨折的专用螺钉对从 THP-1 单核细胞获得的巨噬细胞中白细胞介素 1（IL-1）和白细胞介素 6（IL-6）浓度的影响。将细胞分别与 3D 钛髁突板和专用螺钉（Synthes、Martin、Medartis 制造商）孵育 24 小时和 48 小时。使用 ELISA 法测量 IL-1 和 IL-6 的浓度。与钛板孵育不会导致巨噬细胞中 IL-1（Synthes：0.89-0.86 pg/mg 蛋白；Martin：1.10-0.80 pg/mg 蛋白；Medartis：1.20-0.84 pg/mg 蛋白）和 IL-6（Synthes：16.00-14.00 pg/mg 蛋白，Martin：13.0-10.0 pg/mg 蛋白；Medartis：9.0-12.0 pg/mg 蛋白）的表达显著增加，与在对照条件下孵育 48 小时的 THP-1 巨噬细胞相比。与钛板相比，3D 钛微型板和专用螺钉均未引起 THP-1 巨噬细胞中 IL-1 和 IL-6 表达的任何变化，这对治疗髁突骨折的临床医生来说是一个重要的观察结果。这证实了钛板可以作为人类的安全/中性材料，尤其是考虑到它们对植入后破骨细胞功能和骨重塑过程的显著影响。